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蒌蒿叶总黄酮的响应面法优化微波辅助提取工艺及其抗氧化活性研究

Studies on Microwave-assisted Extraction Technology of Total Flavonoids from the Artemisia selengensis by Response Surface Methodology and Its Antioxidant Activity

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【作者】 扶庆权侯佩柳闽生华春

【Author】 Fu Qing-quan;Hou Pei;Liu Min-sheng;Hua Chun;School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;

【机构】 南京晓庄学院生物化工与环境工程学院中国农业科学院作物科学研究所

【摘要】 以蒌蒿叶为材料,采用微波辅助提取总黄酮。在单因素试验的基础上,通过响应面法,对蒌蒿叶总黄酮的微波辅助提取工艺进行了优化,并对其抗氧化活性进行了研究。结果表明:微波辅助提取蒌蒿叶总黄酮的最佳工艺条件为料液比1︰130(g/mL),乙醇体积分数80%,微波功率440 W,处理时间6 min,在此条件下蒌蒿叶总黄酮得率为5.52%。蒌蒿叶黄酮提取液对DPPH自由基的平均抑制率为86.20%,表现出很强的抗氧化活性,因此,具有较好的综合开发利用前景。

【Abstract】 The optimization of microwave-assisted extraction of the total f lavonoids from Artemisia selengensis was studied. On the basis of design and analysis of single-factor experiments,the technological conditions of microwave-assisted extraction for total f lavonoids from Artemisia selengensis were optimized by response surface methodology,moreover,in vitro antioxidant activity of total f lavonoids from Artemisia selengensi s was also studied by DPPH. The results showed that the optimum extraction conditions of total f lavonoids from Artemisia selengensis were solid/liquid ratio of 1︰130(g/mL),ethanol concentration of 80%,microwave power of 440 W and microwave time of 6 min; futhermore,the extraction rate of total f lavonoids from Ar tem isia sele ngensis was 5.52% under t he optimal conditions; in addition,the average inhibition rate for DPPH on total f lavonoids from Artemis ia selengensis was 86.20%,which revealed stronger antioxidant activity on DPPH,and it will make a good prospect of the comprehensive exploitation and application of Artemisia selengen sis.

【基金】 南京晓庄学院校级应用型研究课题(2011NXY06)
  • 【分类号】TQ461
  • 【被引频次】10
  • 【下载频次】326
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