【摘要】 对胰蛋白酶脱除薤白多糖中蛋白质工艺进行研究。以蛋白脱除率和多糖剩余率及综合评分为考察指标,在单因素试验的基础上通过正交试验设计对工艺进行优化。结果表明,最佳工艺条件为:超声时间35 min,水浴温度30℃,酶液pH 8.5,酶液用量0.80 mL。在此条件下蛋白脱除率为86.86%,多糖剩余率为74.69%。此方法可用于薤白多糖中蛋白质的脱除。更多还原

【Abstract】 The deproteinized process with trypsin from Polysaccharide in Allium macrostemon Bunge was studied. The deproteinized conditions were optimized by single factor and orthogonal array design methods based on deproteinization rate and the residual rate of polysaccharides. The optimal conditions were: ultrasonic time 35 min, the temperature of water bath 30 ℃, pH 8.5, enzyme dosage 0.80 mL. Under this condition the protein removal rate was 86.86%, the polysaccharides residual rate was 74.69%.更多还原