【摘要】 目的利用高压液相色谱法测定东北林蛙输卵管(林蛙油)、中华绒螯蟹卵(蟹黄)以及鲫鱼卵(籽)中雌二醇的含量。方法色谱柱为Agilent Zorbax SB-C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(63:37),检测波长280 nm,流速1.0 mL/min,柱温30℃,进样量为10μL。结果在0.3～10μg/mL浓度范围内,高压液相色谱图峰面积与雌二醇浓度线性关系良好,其回归方程为Y=4203.8X-661.95,相关系数R=0.9991。林蛙油、中华绒螯蟹卵和鲫鱼卵中雌二醇的含量分别为3.00、0.42、0.54μg/g。结论超声波法可以有效提取样品中的雌二醇,通过优化所建立的方法对其具有良好的线性关系和回收率,高压液相色谱峰型美观、分离效果好。更多还原

【Abstract】 Objective To determine the concentration of estradiol in Rana oviduct, ovum of Eriocheir sinensis and ovum of Crucian carp by high pressure liquid chromatography. Methods The chromatographic column Agilent Zorbax SB-C18(4.6 mm×250 mm, 5 μm) was used. Chromatographic conditions were as follows: the mobile phase was acetonitrile-water(63:37), the detection wavelength was 280 nm, the flow rate was 1.0 mL/min, column temperature was 30 ℃, and sample amount was 10 μL. Results A good linearity were obtained in the range of estradiol concentration 0.3～10 μg/mL with the above condition. Its regression equation was Y=4203.8X-661.95 with the correlation coefficient R=0.9991. The estradiol concentration of Rana oviduct, ovum of Eriocheir sinensis and ovum of Crucian carp were 3.00, 0.42, 0.54 μg/g, respectively. Conclusion The estradiol in these samples could be extract effectively by ultrasonic method. The optimized method has a good relationship and recovery rate for estradiol, and its peak pattern and separating effect of HPLC was good.更多还原