【摘要】 目的:观察脾虚型克罗恩病(CD)大鼠结肠粘膜中血管活性肠肽(VIP)及其受体(VIPR1)表达的变化,以及白术茯苓汤不同配比对其的影响。方法:采用破气耗气加饥饱失常之复因脾虚造模法结合Morris方法复制脾虚型CD动物模型,白术茯苓汤不同配比灌胃给药,免疫组化法检测结肠组织VIP及VIPR1的蛋白表达。结果:与空白组相比,模型组大鼠结肠VIP及其受体VIPR1蛋白表达明显增高(P<0.05);与模型组比较,B5组(白术∶茯苓=12∶15)、B6组(白术∶茯苓=15∶12)、B7组(白术∶茯苓=18∶9)结肠组织VIP及其受体VIPR1蛋白表达明显降低(P<0.05)。结论:白术茯苓汤B5组、B6组、B7组剂量配比能下调脾虚型克罗恩病结肠组织VIP及其受体VIPR1蛋白表达,从而防止致炎因子的分泌,减轻结肠的炎症反应,发挥其治疗作用。更多还原

【Abstract】 This study was aimed to observe the effect of Bai-Zhu Fu-Ling(BZFL) Decoction in different proportioning on VIP and VIPR1 in Crohn’s disease(CD) rats with spleen deficiency syndrome, in order to further explore the immunologic mechanism of BZFL Decoction on CD. The CD rat model with spleen deficiency syndrome was established using exhaustion and hunger. The model rats were treated by BZFL Decoction with different proportioning, and immunohistochemistry(IHC) was used to detect the expression of VIP and its receptor in colon tissues. The results showed that comparing to the blank control group, the level of VIP and its receptor of the model group significantly increased(P < 0.05). Comparing to the model group, the level of VIP and its receptor in BZFL Decoction B5 group(Rhizoma Atractylodis Macrocephalae∶Poria = 12∶15), B6 group(Rhizoma Atractylodis Macrocephalae∶Poria = 15∶12)and B7 group(Rhizoma Atractylodis Macrocephalae∶Poria = 18∶9) was significantly decreased(P < 0.05). It was concluded that the effect of BZFL Decoction of B5 group, B6 group and B7 group was better than other groups in VIP and its receptor which can regulate the VIP and its receptor, inhibit the releasing of inflammatory factors and reduce intestinal inflammation injury.更多还原