【摘要】 目的探讨过表达miR-34a对胃癌细胞侵袭转移增殖能力的影响,以及其可能的作用机制。方法通过转染miR-34a mimics上调其表达,利用划痕迁移实验、transwell侵袭实验和CCK8实验检测过表达miR-34a对SGC-7901,BCG-823细胞的迁移、侵袭和增殖能力的影响。采用生物信息学预测及双荧光素酶实验考察miR-34a对靶基因Fra-1的靶向调控机制。结果转染miR-34a mimics过表达miR-34a能显著抑制胃癌细胞SGC-7901,BCG-823的迁移、侵袭和增殖能力。miR-34a能够靶向负性调控Fra-1的表达,影响下游侵袭转移相关蛋白分子MMP9、Cyclin D1的表达。结论过表达胃癌细胞系中miR-34a可靶向抑制Fra-1的表达,从而抑制胃癌细胞的侵袭转移增殖能力,影响胃癌进展。更多还原

【Abstract】 Objective To investigate the effect of miR-34 a on the proliferation,migration and invasion of gastric cancer(GC) cell lines SGC-7901,BCG-823 and find out the potential target genes. Methods MiR-34 a mimics were transfected into two GC cell lines. Wound healing assay,transwell assay and CCK8 test were performed to explore the effect of miR-34 a on the proliferation,migration and invasion of gastric cancer( GC) cell lines. Bioinformatics and Dual luciferase experiment were performed to investigate the potential target genes. Results Up-regulation of miR-34 a could inhibit the proliferation,migration and invasion of SGC-7901,BCG-823 cells. Dual luciferase experiment confirmed that Fra-1 was a potential target gene of miR-34 a,which could reduce the expression of downstream molecular(MMP-9 and Cyclin D1). Conclusion Overexpression miR-34 a could inhibit the proliferation,migration and invasion of GC cell lines through or partly via regulating the target gene Fra-1 and its downstream molecular MMP-9 and Cyclin D1.更多还原