【摘要】 利用RACE方法克隆到长春花中编码黄素单加氧酶的基因(YUCCA),YUCCA(YUC)是植物生长素吲哚乙酸(IAA)合成通路上的关键酶。长春花YUC(CrYUC,GeneBank登陆号KF827426)cDNA序列全长1 863bp,包括编码405个氨基酸的1 218bp开放阅读框、5’非编码区、3’非编码区和poly A尾巴;其相应的DNA中有3个内含子。生物信息学分析结果显示:预测CrYUC的分子量为45.3kDa,等电点为9.01;有16个磷酸化活性位点;与其他物种的YUC有较高相似度。初步表达分析发现:YUC在长春花的叶片、花、根及茎中均有表达,且茎及叶片中表达量高于花及根中表达量。更多还原

【Abstract】 In this study,the YUCCA gene which encodes flavin monooxygenase(named as CrYUC,GeneBank number:KF827426)was cloned from Catharanthus roseus.YUC plays an important role in the auxin biosynthesis.The full length of CrYUCcDNA is 1 863 bp including 1 218 bp open reading frame which encodes 405 amino acids,3’,5’-unencoding area and Poly A tail area.The DNA sequence of CrYUC contains three introns.The results of biovinformation analysis showed that the molecular weight of CrYUC was 45.3and the isoelectric point was 9.01.There was 16 phosphorylation activity sites and CrYUC was homologous with YUCs of other plant species.The RT-qPCR result showed that CrYUCmRNA existed in leaf,flower,stem and root.But the expression level of stem and leaf were higher than that of root and flower.更多还原