【摘要】 将pEGFP-C1质粒与脂质体混合后注入小鼠睾丸内,30d后观察睾丸形态和组织变化;检测精子和配种后仔鼠转基因阳性率,探讨小鼠睾丸网、曲精细管和睾丸间质3种注射方法对转基因小鼠生产效率的影响。结果显示:3种不同注射方法对小鼠睾丸造成损伤由大到小依次为睾丸网注射、曲精细管注射和睾丸间质注射。荧光检测小鼠精子,睾丸网和曲精细管注射法阳性率分别为(35.13±1.72)%和(15.13±1.45)%,睾丸间质注射法没有观察到阳性精子。PCR检测仔鼠,三者的转基因效率分别为33.3%、12.5%和0。更多还原

【Abstract】 The plasmid DNA(pEGFP-C1) mixed with liposome was injected into mouse testes,30days later the morphological and histological changes of testes were observed,and the rates of both positive sperms and positive transgenic mice after joining were determined so as to compare the effects of three testis injection methods(rete testis injection,seminiferous tubules injection and testicular interstitial injection) on the productive efficiency of transgenic mice.The results showed that the descending order of testis damages by the above 3 methods was rete testis injection,seminiferous tubules injection and testicular interstitial injection.The fluorescence detection of mouse perms indicated that the positive sperm rates of rete testis injection,seminiferous tubes injection and testicular interstitial injection were(35.13±1.72)%,(15.13±1.45)%and 0 respectively.The PCR detection of the progeny indicated that the positive transgenic mice rates by the 3 methods were33.3%,12.5%and 0 respectively.更多还原