【摘要】 目的:研究金属蛋白酶解离素28(a disintegrin and metalloproteinase 28,ADAM28)反义核酸(antisense oligodeoxynucleotide,AS-ODN)对人牙龈成纤维细胞(human gingival fibroblasts,HGFs)增殖、分化、凋亡的影响,并分析可能的作用机制。方法:采用细胞培养、基因转染、四甲基唑蓝(MTT)比色法、酶动力学法和流式细胞术(FCM)检测ADAM28反义核酸转染HGFs后对细胞生物学特性的影响。采用SPSS16.0软件包中的SNK检验对数据进行统计学分析。结果:ADAM28 AS-ODN组HGFs的增殖活性显著降低。AS-ODN组HGFs处于S期的细胞百分比显著低于SODN组和未转染组,G2+M期的细胞百分比显著低于未转染组,AS-ODN组的细胞增殖指数(PI=S+G2M)显著低于其他2组,差异显著。AS-ODN组碱性磷酸酶(ALP)分泌活性、凋亡细胞百分比显著升高。结论:ADAM28反义核酸可显著抑制HGFs的增殖,并影响细胞周期的变化,促进其分化,显著诱导HGFs的凋亡。更多还原

【Abstract】 PURPOSE: To study the effects of a disintegrin and metalloproteinase 28(ADAM28) antisense oligodeoxynucleotide(AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts(HGFs), and analyze the possible mechanism. METHODS: Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry(FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package.RESULTS: In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G2+M phase was remarkably lower than that of untransfected group. Cell proliferation index(PI=S+G2M)in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase(ALP) secretion activity and percentage of apoptotic cell notably increased. CONCLUSIONS:ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.更多还原