【摘要】 目的:探讨宫颈上皮内瘤变(CIN)组织中P16蛋白表达及其与高危型人乳头瘤病毒(HR-HPV)病毒载量的相关性。方法:分别采用免疫组化SP法和第二代杂交捕获法(HC-2)检测宫颈(正常宫颈42例,CINⅠ级32例,CINⅡ53例,CINⅢ92例)组织中P16蛋白表达及HR-HPV病毒载量。结果:①从正常宫颈、CINⅠ、CINⅡ到CINⅢ,宫颈组织P16蛋白表达强度及HR-HPV阳性率均递增,差异有统计学意义(P<0.01)。②不同级别CIN组织中HR-HPV病毒载量分别呈正态分布,并随病变级别增高,病毒载量中位数增高,但病毒载量与病变级别之间无相关性(r s=0.1412,P>0.05)。③P16蛋白表达强度与CIN级别呈正相关(r s=0.3633,P<0.01)。④在CIN组织中,P16蛋白表达与HR-HPV病毒载量呈正相关(r s=0.4781,P<0.01)。结论:P16、HR-HPV感染参与了CIN的发生与发展,P16免疫组化检测对宫颈癌前病变的早期诊断有很高的参考价值。更多还原

【Abstract】 Objective: To examine the correlation between P16 and HR-HPV load in cervical intraepithelial neoplasia( CIN) and the potential clinic significance. Methods: Expression of P16 protein was detected by immunohistochemical staining and HR-HPV load was detected by hybrid capture Ⅱ( HC-2) in 177 specimens of CIN( 32 cases of CINⅠ,53 cases of CINⅡ and 92 cases of CINⅢ) and 40 specimens of normal cervix. Results: The positive rates of P16 and HR-HPV in CIN were both significantly higher than those in normal cervix( P<0. 01). The HR-HPV load had a normal distribution in each level of the CIN,and the median value in each level increased with the level increasing. There was no relationship between the HR-HPV load with the CIN level( r s =0. 1412,P>0. 05). P16 expression in CIN increased with the CIN level increasing( r s =0. 3633, P<0. 01). The expression of P16 was positively correlated to the HR-HPV load in CIN( r s = 0. 4781,P < 0. 01). Conclusions: P16 and the infection of HR-HPV contribute to development of CIN,and P16 has some value in early diagnosis of CIN.更多还原