【摘要】 目的:测定不同来源黄芪药材中黄芪甲苷的含量,为黄芪药材的质量评价提供实验依据。方法:采用HPLC-ELSD法,色谱条件:Diamonsil C18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-水(32∶68);流速:1.0 m L·min-1;柱温:30℃。ELSD参数:载气流速2.7 L·min-1;漂移管温度:105℃。结果:黄芪甲苷的线性范围为2.5~12.5μg,r=0.9996(n=6),平均加样回收率为102.40%(RSD1.76%)。结论 :不同产地黄芪药材黄芪甲苷含量存在较大差异。该方法简便快捷,重现性好,可广泛用于黄芪药材的质量控制与评价。更多还原

【Abstract】 Objective:To determine astragaloside Ⅳ in Astragali Radix from different areas by HPLC-ELSD so as to provide experimental basis for quality evaluation of Astragali Radix. Method:HPLC-ELSD was used in the quantitative analysis of astragaloside Ⅳ. The measurement conditions were as follows:Diamonsil C18(250 mm×4.6 mm,5 μm) column,and mobile phase:acetonitrile-water(32∶68),and flow rate:1.0 m L·min-1,and column temperature:30 ℃,and detector:ELSD 2000,and gas flow:2.7 L·min-1,and the temperature of drift tube:105 ℃.Results:The linear range of astragaloside Ⅳ was 2.5 μg~12.5 μg,r=0.9996(n=6),the average recovery was102.40%(RSD1.76%). Conclusion:There were great differences in astragaloside Ⅳ content of Astragali Radix from different areas. The method is of simplicity,accuracy and good reproducibility,and can be widely used in the quality control and evaluation of Astragali Radix.更多还原