【摘要】 目的探讨入肝门静脉完全动脉化(PVA)加门腔分流术(PCS)对肝硬化大鼠肝脏诱导型一氧化氮合成酶(iNOS)水平的影响。方法制作肝硬化大鼠模型100只。随机分为A组40例、B组40例、C组20例。A组行PVA+PCS,B组仅行PCS,C组行门静脉阻断30 min+右肾切除。免疫组化法检测术前及术后1、2、4、8周各组肝组织中的iNOS,采用Image-Pro Plus6.0图像分析软件分析结果。结果三组术后各时点iNOS水平均低于术前(P均<0.05),术后1、2、4周A组iNOS水平高于B、C组(P均<0.01),术后8周三组iNOS水平差异无统计学意义。结论 PVA加PCS可上调肝硬化大鼠肝脏组织中iNOS,但这种变化主要集中在术后4周内。更多还原

【Abstract】 Objective To evaluate the impact of inducible nitric oxide synthase( iNOS) expression following portal vein arterializations( PVA) associated with portocaval shunt( PCS) in cirrhotic rats liver. Methods A total of 100 rats were involved to make cirrhosis model and randomly equally divided into three groups,which were group A( adopted PVA +PCS operation),group B( PCS operation only),group C( adopted portal vein block for 30 mins and right nephrectomy operation). iNOS in liver of each group was measured by method of immunohistochemistryat the moment of pre-operation,postoperative 1 week,2 weeks,4 weeks and 8 weeks. The results were analysed by software Image-Pro Plus 6. 0. Results The values of iNOS decreased obviously over time after surgery in three groups,which were significantly lower than the values of pre-operation( all P < 0. 05). The values of iNOS in group A were significantly higher than the other two groups at the moment of postoperative 1,2 and 4 weeks( all P < 0. 01). Until the moment of postoperative 8 weeks,there was no significant difference between the group A and the other two groups. Conclusions PVA with PCS technique can raise the iNOS expression of liver tissues in cirrhotic rats within postoperative 4 weeks,but this reactions gradually become less obvious after postoperative 4 weeks.更多还原