【摘要】 目的观察血管内皮生长因子(VEGF)-A和VEGF-C在星形细胞肿瘤组织中的表达变化,分析两指标间及与肿瘤血管生成的关系。方法留取93例星形细胞肿瘤患者肿瘤组织及9例接受高血压脑出血开颅手术患者的正常脑组织,用免疫组织化学(SP)方法检测VEGF-A、VEGF-C蛋白表达和微血管密度(MVD),RT-PCR法检测VEGF-A、VEGF-C mRNA表达,对两VEGF间及与肿瘤WHO分级、MVD的关系进行统计学分析。结果星形细胞肿瘤组织中VEGF-A、VEGF-C蛋白阳性表达率及MVD均显著高于正常脑组织,且均随肿瘤恶性程度增高而增强(P均<0.01);星形细胞肿瘤组织中VEGF-A、VEGF-C蛋白表达及两者与MVD均呈显著相关(P均<0.01)。星形细胞肿瘤组织中VEGF-A和VEGF-C mRNA表达均显著高于正常脑组织,且均随肿瘤恶性程度增高而增强(P均<0.05);肿瘤组织中VEGF-A和VEGF-C mRNA表达呈显著相关(P均<0.05)。结论 VEGF-A、VEGF-C在星形细胞肿瘤组织中的表达上调并与肿瘤恶性程度有关,两者间及与肿瘤血管生成均关系密切。更多还原

【Abstract】 Objective To observe the expression changes of vascular endothelial growth factor-A( VEGF-A) and vascular endothelial growth factor-C( VEGF-C) in low-grade and high-grade astrocytic gliomas and their correlation with angiogenesis,which will provide the basis for target therapy and prognosis of astrocytomas. Methods Ninety-three astrocytic glioma samples and 9 normal brain tissues from spontaneous intracranial hemorrhage were obtained. The protein expression and microvessel density( MVD) of VEGF-A and VEGF-C was examined with immunohistochemical staining method( S-P). The mRNA expression of VEGF-A and VEGF-C was examined with fluorescent quantitative polymerase chain reaction( RT-PCR). Statistical analysis was employed to determine the reciprocal correlation between VEGF-A and VEGF-C,and their correlation with WHO grades and MVD. Results The positive expression rates of VEGF-A,VEGF-C,and MVD in the astrocytic tumor tissues were significantly higher than those of the normal brain tissues,and the degree of expression was in accordance with the malignance of tumors( all P <0. 01). The expression of VEGF-A,VEGF-C protein was correlated with MVD( all P <0. 01). Meanwhile,the expression of VEGF-A,VEGF-C mRNA was higher than that of the normal tissues and the degree of expression was in accordance with the malignance of tumors( all P <0. 05),and the VEGF-A mRNA was significantly correlated with VEGF-C mRNA( all P < 0. 05).Conclusion The expression of VEGF-A and VEGF-C is up-regulated and is correlated with the malignancy of astrocytic tumor,which shows a close correlation between each other as well as their correlation with tumor angiogenesis.更多还原