Objective To prepare the antibody specifically against human tumor necrosis factor-α induced protein 8 like 3( TIPE3) and investigate the feasibility and specificity of the antibody in detecting TIPE3 protein in human tissues. Methods The specific polyclonal antibody was prepared by immunization of rabbits with TIPE3 antigen. Human TIPE3 eukaryotic expression vector w as constructed. The specificity of TIPE3 antibody and the expression of TIPE3 protein in tumor cell lines w ere analyzed by Western blotting....
【更新日期】
2014-05-06
【分类号】
R392.11
【正文快照】
肿瘤坏死因子-α诱导蛋白8(tumor necrosisfactor-α-induced protein 8,TIPE或称TNFAIP8)家族是近年发现的、在免疫自稳和肿瘤生长中发挥调节作用的一组蛋白质[1]。TIPE家族包括TIPE、TIPE1、TIPE2和TIPE3四个成员,它们的结构具有高度同源性,均包含7个高度保守的α螺旋(α0~α
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