【摘要】 目的观察醒酒方的醒酒防醉作用并探讨其作用机制。方法建立小鼠乙醇急性中毒模型,随机分为正常对照组、模型组、阳性对照组、醒酒方低、中、高剂量组(3,6,12 g生药/kg),每组12只,观察各组小鼠的攀附时间、醉酒潜伏时间、持续醉酒时间、醉酒率、死亡率以及自主活动次数变化,以评价醒酒方的醒酒防醉作用。同时检测小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乙醇脱氢酶(ADH)的活性以及肝脏中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,并观察其对肝组织形态的影响。结果醉酒后给予醒酒方药物,其各剂量组均不同程度地缩短醉酒小鼠的醉酒潜伏时间,降低醉酒率;对于持续醉酒时间,只有高剂量组有缩短作用(P<0.05);其中高和中剂量组对酒精致小鼠自主活动次数降低的情况均有改善作用;醒酒方各剂量组可明显降低小鼠血清ALT、AST活性及提高ADH活性(P<0.05),升高小鼠肝组织SOD活性并降低其MDA含量(P<0.05);病理结果表明,醒酒方各剂量组对由乙醇引起的肝细胞变性、坏死,炎细胞浸润均有明显改善。结论醒酒方有显著的醒酒作用,这可能与其提高ADH、SOD活性,降低MDA含量等抗氧化作用有关。更多还原

【Abstract】 Objective To study the anti-alcoholism effect and its mechanism of Xingjiu Prescription. Methods Alcoholism model in mice was established with 50 % alcohol. The 72 male mice were randomly divided into 6 groups: normal group, model group, Haiwang Jinzun positive group, low-dose Xingjiu Prescription group(3 g crude drug/kg), medium-dose group(6 g crude drug/kg), high-dose group(12 g crude drug/kg), 12 mice in each group. The climbing time, the latent time of drunkenness, the durative time of drunkenness, the rate of drunkenness and death, the change of spontaneous activity frequency of mice were observed in all groups to evaluate the anti-alcoholism effect of Xingjiu Prescription. The activities of AST, ALT, ADH in serum, and the activity of SOD and the content of MDA in the liver were determined. The liver tissue morphology was also observed. Results Compared to model group, all dosages of Xingjiu Prescription could significantly reduce the rate of drunkenness, shorten the latent time of drunkenness(P<0.05) when administered after drunkenness. Only the high dosage of Xingjiu Prescription significantly reduced the durative time of drunkenness(P<0.05). Both medium-dose and high-dose of Xingjiu Prescription could increase the frequency of spontaneous activity of mice(P<0.05). All dosages of Xingjiu Prescription could significantly down-regulate the levels of ALT, AST and MDA(P<0.05), and up-regulate the levels of ADH and SOD(P<0.05), compared with the model group. Pathology results showed that the severe hepatic lesions as well as neutrophilic infiltration and ballooning degeneration of hepatocytes induced by ethanol were considerably reduced in Xingjiu Prescription treated groups. Conclusion Xingjiu Prescription has a significant anti-alcoholism effect, which may be associated with the increase of the activities of ADH, SOD and decrease of the content of MDA.更多还原