【摘要】 为保护野生资源,以射干幼茎为材料,应用植物组织培养技术,进行了愈伤组织培养、愈伤组织分化培养、试管苗培养和试管苗的移栽与定植的研究。结果显示,幼茎段培养愈伤组织的适宜培养基是MS+琼脂5.3 g·L-1+蔗糖38 g·L-1+2,4-D2.2 mg·L-1+6-BA 0.8 mg·L-1;愈伤组织分化无根苗培养的适宜培养基是1/2MS+琼脂5.3 g·L-1+蔗糖42 g·L-1+NAA0.05 mg·L-1+6-BA 1.4 mg·L-1;试管苗培养的适宜培养基是1/4MS+蔗糖10 g·L-1+琼脂5.3 g·L-1+IBA0.05 mg·L-1+IAA0.25 mg·L-1;试管苗移栽成活率为90.3%;定植成活率为96.0%。定植试管苗翌年正常开花结果,野生的射干的植物性状保持不变。更多还原

【Abstract】 We addressed callus culture,differentiation of callus culture,test-tube seedling culture and transplanting and colonization of test-tube seedling with the younger stems of Belamcanda chinensis as materials and plant tissue culture technology to protect wild-life resources. Results demonstrate that MS + agar 5. 3 g ·L- 1+ sucrose 38 g ·L- 1+ 2,4-D2. 2 mg·L- 1+ 6-BA 0. 8 mg·L- 1is the suitable medium of callus culture,1 /2MS + agar 5. 3 g·L- 1+ sucrose 42 g·L- 1+NAA 0. 05 mg · L- 1+ 6-BA 1. 4 mg · L- 1suitable for the differentiation of rootless seedling culture,1 /4MS + sucrose10 g·L- 1+ agar 5. 3 g·L- 1+ IBA 0. 05 mg·L- 1+ IAA 0. 25 mg·L- 1appropriate for test-tube seedling culture. Survival rate of test-tube seedling transplanting is 90. 3% and its colonization survival rate is 96. 0%. Test- tube seedling blossoms and bears fruit in the following year and maintains all biological traits of wild plant.更多还原