【摘要】 以L-酒石酸正己酯-硼酸配合物为流动相添加剂,采用反相高效液相色谱法分离了班布特罗和妥洛特罗2种手性β-受体激动剂。实验考察了酒石酸正己酯和硼酸的浓度,缓冲溶液的种类、浓度、pH值,有机改性剂甲醇的含量对手性分离效果的影响。在最佳手性分离条件下,班布特罗和妥洛特罗均可以达到基线分离。更多还原

【Abstract】 The di- n- hexy L- tartrate- boric acid complex was used as a chiral mobile phase additive for the enantioseparation of two β- agonist including bambuterol and tulobuterol by reversed- phase high performance liquid chromatographic( RP- HPLC). The influences of concentrations of di- n- hexy L- tartrate and boric acid,the type,concentration and pH of the buffer,as well as methanol content were extensively investigated. Under the optimized conditions,baseline enantioseparations were both obtained for the two analytes.更多还原