【摘要】 本研究以屠宰奶山羊的新鲜卵巢为材料,回收可培养卵丘细胞-卵母细胞复合体(COC)。将可培养COC置于M199+FBS+100 IU/mL FSH+100 IU/mL LH培养微滴中,在5%CO2、95%空气、饱和湿度的CO2培养箱中39℃下培养。进行二因子三水平设计,比较基础培养液中添加FBS体积分数为0%、10%和15%时,及培养时间16 h、24 h、26 h的成熟率。结果表明:不添加FBS组的成熟率极显著(P<0.01)低于其他两组,而10%FBS组与15%FBS组差异不显著(P>0.05);体外培养16 h与培养24 h、26 h的卵母细胞成熟率分别为67.6%与81.7%、81.7%,差异极显著(P<0.01),而后二者间未表现差异。更多还原

【Abstract】 This experiment regarded fresh ovary from slaughtered goats as the material. This kind of COC was placed in M199 + the FBS( 0%,10%,15%) educates liquid + FSH( 100 IU /mL) + LH( 100 IU /mL) educates tiny inside,in the CO2incubator( 5% CO2,95% air,100% humidity) under 39℃ temperature descends to educate 16 h,24 h and 26 h. It is concluded that 10% ~ 15% FBS is beneficial to the maturation in vitro of goat follicular oocytes. The maturation rate of oocytes cultured for 24 h,26 h was significantly higher than that of 16 h( P < 0. 05).更多还原