【摘要】 目的探讨用微柱凝胶抗人球蛋白试验检测的孕妇血型抗A(B)免疫球蛋白G(IgG)效价与ABO新生儿溶血病(HDN)发生率的关系。方法应用微柱凝胶技术对黄疸HDN可疑的新生儿进行肝功能、血型及HDN试验检测,同时对其母亲为O型RhD阳性的进行IgG抗A(B)抗体效价检测。结果在149例患者中,18例母亲IgG效价≤64的,新生儿均未发生HDN;母亲效价为128、256、512、≥1 024时,HDN的发病率分别为12.0%、75.0%、87.5%、100.0%;随着抗体效价的提高,HDN发病率逐步升高,二者呈正相关(r~2=0.831,P=0.005)。母亲IgG抗体效价≥256与<256时,HDN的发病率差异有统计学意义(χ~2=14.896,P<0.05)。结论母亲的抗体效价用微柱法检测在128时发生HDN的几率仅有12.0%,且症状程度也很轻,远低于效价256时的75.0%,因此认为微柱凝胶卡检测孕妇IgG抗体效价的临界值可定为256。更多还原

【Abstract】 Objective To investigate the relationship between anti A(B) immunoglobulin G(IgG) titer detected by antiglobulin test with microcolumn gel and ABO hemolytic disease of newborn(HDN).Methods Microcolumn gel technique was used to detect liver function,blood type and HDN test on suspected neonatal jaundice HDN.At the same time,the mothers with 0 type of positive RhD were detected for IgG anti A(B) antibody titer.Results In 149 patients,there were 18 cases of maternal with IgG titer ≤64,and HDN didn’ t happen on their newborns.When maternal IgG titer was 128,256,512 and ≥1 024,the incidence of HDN was 12.0%,75.0%,87.5%and100.0%,respectively.With the enhancement of antibody titer,HDN incidence increased gradually.They were positively correlated(correlation coefficient r2 =0.831,P = 0.005).The incidence of HDN was significantly different when maternal IgG antibody titer≥256and <256(x~2 =14.896,P<0.05).Conclusion The incidence of HDN is only 12.0%when maternal antibody titer is 128 detected by microcolumn gel,and symptoms are mild,which is significantly lower than 75.0%when maternal antibody titer is 256.So the critical value of maternal IgG antibody titer detected by microcolumn gel card can be set to 256.更多还原