【摘要】 【目的】观察鼻窦灌注液冲洗鼻腔对鼻息肉相关的细胞因子水平的影响。【方法】将39例鼻息肉患者随机分为鼻窦灌注液组18例和生理盐水组21例。全部鼻息肉术后患者在接受术后综合治疗同时,分别采用鼻窦灌注液、9 g/L生理盐水冲洗鼻腔3个月。采用鼻腔鼻窦结局测试-20(SNOT-20)及Lund-Kennedy评分评估疗效,术前及术后3个月分别取鼻腔中鼻道黏膜作标本,采用酶联免疫吸附(ELISA)法检测黏膜中白细胞介素-5(IL-5)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)、干扰素-γ(IFN-γ)、粒细胞—巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子-α(TNF-α)的表达水平。【结果】(1)术后2组SNOT-20及Lund-Kennedy评分均较术前降低,差异有统计学意义(P<0.05),但术后2组比较,差异均无统计学意义(P>0.05)。(2)术后3个月,2组黏膜细胞因子IL-5、IL-8、IL-10、IFN-γ、TNF-α、GM-CSF表达水平均较术前显著降低(P<0.05),且鼻窦灌注液组对细胞因子IL-5、IL-8、IL-10、IFN-γ表达水平的降低作用优于生理盐水组(P<0.05),而2组TNF-α、GM-CSF的表达水平比较,差异无统计学意义(P>0.05)。【结论】术后3个月,2组在改善患者生活质量及鼻腔形态方面疗效相当,而鼻窦灌注液对IL-5、IL-8、IL-10、IFN-γ水平的调节作用优于生理盐水。更多还原

【Abstract】 Objective To observe the influence of nasal washing with Bidouguan Perfusate(BP) on nasal mucosal cytokines of nasal polyp patients after operation. Methods Thirty-nine nasal polyp patients were randomized into BP group( N =18) and normal saline group( N =21). All patients received operation for nasal polyp and postoperative comprehensive treatment, and additionally, BP group was given washing with BP and normal saline group was washed with 0.9% normal saline for 3 months. The scores of SNOT-20 and Lund-Kennedy were observed for the evaluation of therapeutic effect. Enzyme-linked immunosorbent assay( ELISA) was used for detection of interleukin 5(IL-5), interleukin 8(IL-8), interleukin 10(IL-10), interferon gamma(IFN-γ), granulocyte-macrophage colony-stimulating factor(GM-CSF), tumor necrosis factor alpha(TNF-α) in nasal mucosa before operation and 3 months after operation. Results(1) SNOT-20 and Lund-Kennedy scores were decreased after operation( P <0.05 compared with those before operation), the difference being insignificant between the two group after operation(P>0.05).(2) The expression levels of IL-5, IL-8, IL-10, IFN-γ, TNF-α and GM-CSF in the nasal mucosa were decreased in both groups 3 months after operation(P<0.05), and the decrease of IL-5, IL-8, IL-10 and IFN-γ in BP group was superior to that in the normal saline group( P <0.05). The differences of TNF-α and GM-CSF levels were insignificant between the two groups after operation( P >0.05). Conclusion Nasal washing with BP has similar effect to normal saline on improving the quality of life and nasal mucosal histology 3 months after operation, but BP has better effect on regulating nasal mucosal IL-5, IL-8, IL-10 and IFN-γ.更多还原