【摘要】 【目的】探讨白芍总苷对大鼠腹膜纤维化转化生长因子-β1(TGF-β1)及其信号转导分子7(Smad7)的干预作用及其机制。【方法】采用42.5 g/L含糖透析液+脂多糖法复制腹膜纤维化模型。将SD大鼠随机分成4组:空白组,模型组,白芍总苷高、低剂量组。第30天后,测定各组腹膜功能,取腹膜组织进行Masson染色观察腹膜病理学变化,采用实时荧光聚合酶链反应(Real-time PCR)方法检测各组腹膜TGF-β1 mRNA、Smad7 mRNA的表达。【结果】模型组腹膜厚度显著增加,腹膜超滤量显著降低(均P<0.05)。白芍总苷高、低剂量组对上述病变均有显著改善作用(P<0.05)。模型组腹膜TGF-β1 mRNA、Smad7 mRNA表达水平显著高于空白组(P<0.05或P<0.01),白芍总苷高、低剂量组TGF-β1表达较模型组均显著降低(P<0.05),白芍总苷高剂量组抑制性信号蛋白Smad7则显著升高(P<0.05)。【结论】白芍总苷抑制腹膜组织TGF-β1 mRNA表达、上调Smad7 mRNA表达可能是其抑制腹膜纤维化的机制之一。更多还原

【Abstract】 Objective To investigate the effect of total glucosides of paeony(TGP) on transforming growth factor(TGF)-β1/Smad7 signal transduction pathway in rats with peritoneal fibrosis and to explore its possible mechanism. Methods The model of peritoneal dialysis fibrosis was established by giving dialysis fluid containing 42.5g/L sugar and lipopolysaccharide. And then the model rats were randomly divided into four groups, blank group, model group, and high- and low-dose TGP groups. After treatment for 30 days, peritoneal function in all of the groups was measured. Peritoneal tissue was stained by Masson for observing the pathological changes. TGF- β1 and Smad7 mRNA expression levels in peritoneal were detected by real-time quantitative polymerase chain reaction(PCR). Results The thickness of the peritoneum was significantly increased and the peritoneal ultrafiltration was the worst in the model group(P<0.05), while the above changes were improved in high-and low-dose TGP gr oups(P<0.05). TGF-β1 and Smad7 mRNA expression levels in the model group were significantly higher than those in the blank group(P<0.05 or P<0.01). TGF-β1 was significantly lowered in high- and low- dose TGP groups and Smad7 was increased in high-dose TGP group(P<0.05 compared with those in the model group). Conclusion TGP can inhibit peritoneal fibrosis induced by peritoneal dialysis and improve peritoneal structures, and one of its possible mechanisms is related with the inhibition of TGF-β1 and the increase of Smad7 mRNA expression in the peritoneal tissue.更多还原