【摘要】 目的在检测不同剂量碘-131对小鼠胸腺细胞自发掺入3H-TdR的基础上,观察碘-131对小鼠体外胸腺细胞自发掺入3H-TdR的影响。方法给予不同剂量碘-131组的小鼠胸腺细胞同体积的3H-TdR,采用3H-TdR自发掺入法检测各组胸腺细胞每分钟计数率(CPM)。结果终浓度为5 Bq/ml的碘-131组小鼠胸腺细胞自发掺入3H-TdR的CPM值显著高于正常胸腺细胞,终浓度为5×105Bq/ml和5×106Bq/ml组小鼠胸腺细胞自发掺入3H-TdR的CPM值明显低于正常胸腺细胞。结论低剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR具有显著的刺激作用,高剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR有明显的抑制作用。更多还原

【Abstract】 Objective In the detection of different doses of131 I on the spontaneous incorporation of3H- TdR in the mice thymocytes in vitro,to observe the effect of131 I on the spontaneous incorporation of3H- TdR into mice thymocytes. Methods The same amount of3H- TdR was given for the different spontaneous incorporation of3H- TdR into thymocytes. Results Experiment in vitro shows that the value of CPM of the spontaneous incorporation of3H- TdR into thymocytes in131I( final concentration is 5Bq / ml) group is markedly higher than that of the normal thymocytes group. The value of CPM of the spontaneous incorporation of3H- TdR into thymocytes in131I( final concentration,5 × 105 Bq / ml and 5 × 106 Bq / ml) group is lower than that of the normal thymocytes group. Conclusion Low dose of131 I had the stimulating effect in the spontaneous incorporation of3H- TdR into thymocytes in vitro and large dose of131 I can significantly inhibit the spontaneous incorporation of3H- TdR into thymocytes in vitro.更多还原