【摘要】 目的探讨Foxp3-6054,-3279基因多态性与子痫前期的相关性。方法采用扩增阻滞突变系统-聚合酶链反应(ARMS-PCR)技术检测156例子痫前期孕妇和252例正常妊娠健康孕妇的外周血Foxp3-6054,-3279基因多态性,并对结果进行统计分析。结果子痫前期孕妇外周血Foxp-3279位点基因型和单基因频率与对照组比较,无显著性差异(P>0.05)。在Foxp3-6054基因多态性中,TT基因型可减少罹患子痫前期的风险,相对危险系数RR=0.734,95%可信区间(95%CI)为0.562~0.960,χ2=5.439,P<0.05;而合并的TA+AA基因型会显著增加罹患子痫前期的风险,RR=1.206,95%CI为1.035~1.405,χ2=5.439,P<0.05。子痫前期孕妇Foxp3-6054与-3279复合基因型中,Foxp3-6054 AA/-3279 CC复合基因型频率明显高于对照组(P<0.05),相对危险系数RR=2.350;而Foxp3-6054 TT/-3279 CC复合基因型频率明显低于对照组(P<0.05),RR=0.748。结论孕妇外周血Foxp-3279基因多态性与子痫前期无关,而Foxp3-6054基因多态性与子痫前期显著相关。更多还原

【Abstract】 Objective To investigate the distribution of-6054,-3279 genes in a Guangdong population and to explore the association of these genes with Pre-eclampsia. Methods 252 unrelated healthy individuals of Han nationality and 156 patients with Pre-eclampsia who were from the same geographic region were enrolled in the study. The Foxp3-6054,-3279 was genotyped by ARMS-PCR technique. Results There was no significant difference between Foxp3-3279 genetypes. The frequency of the-6054 TT genotypes was significantly among Preeclampsia patients than among the controls. There was no significant difference in each allele between Preeclampsia group and control group,the Foxp3-6054 AA /-3279 CC haplotype frequency in patients with Preeclampsia was significantly higher than that in controls( P < 0. 01). The Foxp3-6054 TT /-3279 CC haplotype frequency in patients with Pre-eclampsia was significantly lower than that in controls( P < 0. 01). Conclusions Foxp3-6054 polymorphisms is associated with Pre-eclampsia.更多还原