【摘要】 目的:探讨肺癌组织中K-ras蛋白与Bcl-2蛋白的表达意义及其相关性。方法:选取2005-01-01-2012-06-30在苏州市立医院本部手术切除并经病理确诊的99例肺癌石蜡标本,应用组织芯片和免疫组化法观察K-ras及Bcl-2蛋白在肺癌组织中的表达,分析其表达相关性及与患者临床因素的关系。结果:K-ras和Bcl-2蛋白在肺癌组织中的表达阳性率分别为25.3%(25/99)和26.3%(26/99)。K-ras蛋白的表达与性别、病理类型及分化程度差异有统计学意义,P<0.05;与年龄、有无淋巴结转移及TNM分期等因素差异无统计学意义,P>0.05。Bcl-2蛋白的表达与年龄、性别、病理类型、分化程度、有无淋巴结转移及TNM分期等因素之间差异均无统计学意义,P>0.05。K-ras与Bcl-2蛋白表达呈显著正相关,rs=0.657,P<0.01。结论:K-ras与Bcl-2蛋白可能参与肺癌的发生发展,并且存在相互促进作用。更多还原

【Abstract】 OBJECTIVE:To investigate the expression and significance of K-ras and Bcl-2proteins in lung cancer and the relationship between them.METHODS:Ninety-nine primary lung cancer tissues samples were obtained from patients at the Suzhou Hospital Affiliated to Nanjing Medical University from January 1st 2005to June 30th 2012.The expression of K-ras and Bcl-2proteins in 99specimens of lung cancer were detected by tissue microarray and immunohistochemistry. The correlations were analyzed between K-ras,Bcl-2and gender,age,histological types,differentiation,classification of lung cancer and lymph node metastasis.RESULTS:The positive expression rates of K-ras and Bcl-2in samples of lung cancer were 25.3%(25/99)and 26.3%(26/99)respectively.K-ras expression was related to gender,histological types, and differentiation of lung cancer(P<0.05).No significant correlation was found between K-ras expression and age,classification of lung cancer and lymph node metastasis(P>0.05).No significant correlation was found between Bcl-2expression and age,gender,histological types,classification and differentiation of lung cancer and lymph node metastasis(P>0. 05).Furthermore,there was obviously positive correlation between K-ras and Bcl-2(rs=0.657,P<0.01).CONCLUSIONS:K-ras and Bcl-2may contribute to the tumorogenesis and development of lung cancer.And there may exist some kind of interaction between K-ras and Bcl-2in lung cancer.更多还原