【摘要】 目的探讨吸烟慢性阻塞性肺疾病(COPD)稳定期患者、健康吸烟者及健康非吸烟者外周血中白介素(IL)-18及Treg细胞变化及其临床意义。方法选择2012年11月—2013年5月于河北省人民医院呼吸内科门诊就诊的吸烟COPD患者33例为吸烟COPD组,体检中心体检健康者分为健康吸烟组19例和健康非吸烟组16例。采用酶联免疫吸附测定(ELISA)法检测受试者外周血中IL-18、IL-17水平,用流式细胞术检测CD8+及Treg细胞比例。结果吸烟COPD组IL-18、IL-17、CD8+较健康吸烟组、健康非吸烟组均升高,Treg细胞较健康吸烟组下降、较健康非吸烟组升高(P<0.05);健康吸烟组IL-18、IL-17、CD8+及Treg细胞较健康非吸烟组均升高(P<0.05)。结论IL-18分泌增多,诱导IL-17、CD8+细胞上升,超出Treg细胞的抑制能力以及Treg细胞的反应钝化可能导致吸烟COPD患者气道的慢性炎症。更多还原

【Abstract】 Objective To explore changes and clinical significances of IL-18 and Treg in peripheral blood of patients with stable chronic obstructive pulmonary disease(COPD),healthy smokers and healthy control non- smokers.Methods 33 smoker patients with COPD(COPD group),19 healthy smokers(healthy smoker group) and 16 healthy non- smokers(healthy non- smoker group) admitted to the Department of Respiratory Medicine of Hebei General Hospital from November 2012 to May 2013 were selected.ELISA method was used to determine IL- 18 and IL- 17 in peripheral blood,and flow cytometry method was used to determine CD8 +and Treg.Results The IL- 18,IL- 17 and CD8 +in COPD group was higher than the healthy smoker group and healthy non- smoker group,and Treg in COPD group was lower than the healthy smoker group,but higher than the healthy non- smoker group(P < 0.05).The IL- 18,IL- 17,CD8 +and Treg were higher in healthy smoker group than the healthy non- smoker group(P < 0.05).Conclusion Patients with COPD have an increase in IL- 18,IL- 17,CD8 +cells with a blunted Treg cells response,resulting in chronic airway inflammation.更多还原