【摘要】 目的观察高效抗逆转录病毒疗法(HAART)治疗24周后获得性免疫缺陷综合征(AIDS)患者辅助性17(Th17)细胞和调节性T(Treg)细胞表达水平的变化。方法选取HAART治疗的45例AIDS患者,将其按CD4+T细胞计数分成1~100 cells/μl、101~200 cells/μl和201~350 cells/μl三组,于抗病毒前和抗病毒24周后采集静脉血,运用流式细胞仪检测外周血中CD4+T细胞、Th17和Treg细胞的表达水平,并比较分析其相互关系。结果 AIDS患者治疗24周时Th17细胞在CD4+T细胞中的表达频率为(1.91±0.84)%,而治疗前为(1.31±0.45)%,差异有统计学意义(t=3.67,P<0.05);Treg细胞表达频率在治疗24周时和治疗前分别为(5.97±0.93)%和(8.32±1.12)%,差异有统计学意义(t=3.32,P<0.05)。治疗24周时,Th17细胞明显升高,CD4+T细胞基数为201~350 cells/μl患者组其Th17细胞表达频率显著高于101~200 cells/μl和1~100 cells/μl组患者(t分别=2.68、4.51,P均<0.05)。Treg细胞表达频率经治疗后显著下降,CD4+T细胞基数为201~350 cells/μl组患者Treg细胞表达频率下调程度显著高于101~200 cells/μl和1~100 cells/μl组患者(t分别=2.47、3.89,P均<0.05)。治疗前CD4+T细胞数与Th17表达的百分率呈正相关(r=0.48,P<0.05),与Treg表达的百分率呈负相关(r=-0.30,P<0.05)。结论 AIDS患者Th17表达水平下降,Treg表达则上升;HAART治疗能有效地上调Th17表达频率,下调Treg细胞水平;CD4+T细胞基数高的患者较基数低的患者对抗病毒治疗其免疫反应更有效,提示Th17和Treg细胞表达频率可作为HAART治疗有效性的一项判断依据。更多还原

【Abstract】 Objective To observe the expression levels of Th17 and Treg cells of acquired immune deficiency syndrome(AIDS) suffered patients after 24 weeks highly active antiretroviral treatment(HAART). Methods According to the CD4+T cell amounts, 45 AIDS patients received HAART treatment were divided into three groups. Flow cytometry technology was used to detect the CD4+T Cells count, Treg and Th17 cells in peripheral blood at the time of pre-therapy and 24weeks. Results The ratio of Th17 cells in AIDS patients was(1.91±0.84)% in 24 weeks, and was(1.31±0.45)% at the time of pre-therapy, the difference was significant(t=3.67, P<0.05). The expression level of Treg was(5.97±0.93) % and(8.32±1.12) % at the time of 24 weeks treatment and pre-therapy with statistical significance(t=3.32,P<0.05). Th17cells expression level was raised after 24 weeks, and which of CD4+T cells for 201~350 cells/μl group showed a remarkable decline than those of CD4+T cells for 101~200 cells/μl and 1~100 cells/μl groups(t=2.68,4.51,P<0.05).Treg cells expression level were down-regulated after 24 weeks treatment, and the dropped levels with of CD4 +T cells for 201 ~350cells/μl group was higher than those of two other groups(t=2.47,3.89,P<0.05). Th17 cells was significantly positively related to the percentage of CD4+T cells count(r=0.48, P<0.05);the correlation of Treg expression and CD4+T Cells count was significantly negative(r=-0.30, P<0.05) at the time of pre-therapy. Conclusions The expression level of Th17 cells decreased,while Treg cells was increased in AIDS patients; HAART treatment could upregulated Th17 cells level and downregulated Treg cells. The patients with higher baseline of CD4+T cells has higher immune reaction effective than thatof the lower baseline CD4+T cells. The Th17 and Treg cells are likely to be the effective indexes to observe HAART progress.更多还原