【摘要】 目的 探讨体外受精(IVF)技术中精子处理前后常规参数与受精率的关系。方法 选择2011年6月到2013年3月在南华星辉生殖健康专科医院行IVF助孕的285个鲜胚周期,将这285个周期分为受精率≤40%组(A组)和受精率>40%组(B组),分别比较两组处理前无A级精子比例、上游法回收率≤5%比例、处理后前向运动(PR)总数<1.0×10~6比例、精子巴氏染色正常形态<4%比例、精子顶体酶活性<48.2μIU/10~6精子比例的差异。结果 A、B两组处理前无A级精子比例(分别为14.7%和0.4%,P<0.05)、上游法回收率≤5%比例(分别为23.5%和0.8%,P<0.05)、处理后PR,总数<1.0×10~6比例(分别为17.6%和0%,P<0.05)均具有显著性差异(P<0.05);精子巴氏染色<4%(分别为11.8%和10.0%)、顶体酶活性<48.2μIU/10~6精子(5.9%和2.4%)均无显著性差异。结论 处理前无A级精子、上游法回收率≤5%和处理后PR总数<1.0×10~6是影响受精率的高危因素;精子巴氏染色<4%和精子顶体酶活性低对预测IVF受精率具有一定局限性。更多还原

【Abstract】 Objective To study the relationship between the general parameters of sperm and the fertilization rate of IVF.Methods A total of 285 IVF cycles were collected from Nanhua Xinghui Reproductive Health Hospital from June 2011 to March 2013.The 285 cycles were divided into group A(the fertilization rate of IVF≤40%)andgroup B(the fertilization rate of IVF >40%).The percentage of without A grade sperm before preparation,the percentage of the recovery of sperm swim-up≤5%,the percentage of the total PR sperm after preparation<1.0×10~6,the percentage of normal sperm morphology of papanicolaous stain<4%and sperm acrosin activity<48.2MIU/10~6 were analyzed respectively.Results Between the two groups there were significant difference in the percentage of without A grade sperm before preparation(14.7%VS.0.4%,P<0.05),the percentage of the recovery of sperm swim-up≤5%(23.5%VS.0.8%,P<0.05),the percentage of the total PR after preparation<1.0×10~6(17.6%VS.0%,P<0.05),and there were not significant changes in the percentage of normal sperm morphology of papanicolaous stain<4%(11.8%VS.10.0%),the percentage of sperm acrosin activity <48.2MIU/106(5.9%VS.2.4%).Conclusion Without A grade sperm before preparation,the recovery of sperm swim-up≤5%and the total PR after preparation <1.0×10~6 were significantly associated with the fertility rate of IVF.Whereas,sperm morphology ofpapanicolaous stain and sperm acrosin activity showed some limition in predicting the fertility rate of IVF.更多还原