【摘要】 目的:探讨季节、血型及精液参数等对捐精者精子冷冻复苏率的影响。方法:回顾性分析陕西省人类精子库捐精者4 088份精液标本,研究季节、血型、禁欲时间、精液量、精子形态、冷冻前精子活力及浓度对精子冷冻复苏率的影响。结果:捐精者精子冷冻复苏率随着精子浓度增高而增加,相关性分析提示精子浓度与冷冻复苏率呈正相关(r=0.247,P<0.01)。而精子冷冻前活力和精子冷冻复苏率呈负相关(r=-0.262,P<0.01)。禁欲第6天组的精子冷冻复苏率[(70.2±5.4)%]明显高于其他禁欲时间组(P<0.01)。精子正常形态率>20%组的精子冷冻复苏率[(71.4±5.1)%]要高于其他各组(P<0.01)。A型血精子冷冻复苏率明显高于B型血[(69.1±4.8)%vs(69.8±4.7)%,P<0.01];季节、精液量与精子冷冻复苏率之间无明显相关性(P>0.05)。结论:捐精者的精子浓度、活力、形态及禁欲时间对于预测精子冷冻复苏率有一定的价值,而季节、血型、精液量与捐精者精子冷冻复苏率无明显相关性。更多还原

【Abstract】 Objective: To investigate the influence of seasons,blood types and semen parameters on the cryosurvival rate of frozen-thawed spermatozoa from sperm donors. Methods: We retrospectively analyzed the influence of seasons,blood types,abstinence period,semen volume,sperm morphology,and pre-freeze sperm motility and concentration on the cryosurvival rate of frozen-thawed sperm in 4 088 semen samples from Shaanxi Human Sperm Bank. Results: The cryosurvival rate of the post-thaw sperm was correlated negatively with the progressive motility of the pre-freeze sperm( r =- 0. 262,P < 0. 01),but positively with pre-freeze sperm concentration( r = 0. 247,P < 0. 01),and it was significantly higher in the 6-day abstinence group( [70. 2 ± 5. 4]%) than in the other abstinence groups( P < 0. 01),so was it in the normal sperm morphology rate > 20% group( [71. 4 ± 5. 1]%) than in the others( P < 0. 01). The survival rate of the post-thaw sperm was not correlated with seasons,blood types,and semen volume( P >0. 05). Conclusion: Sperm motility,concentration and morphology and the abstinence period of sperm donors may be valuable predictors of the cryosurvival rate of post-thaw sperm,but no correlation was found between the survival rate and seasons,blood types and semen volume.更多还原