【摘要】 目的:分析具有透明质酸受体精子的质量特征,寻找精子质量评估新指标。方法:用透明质酸包被载玻片检测活动精子-透明质酸结合率,分析其与精液常规、精子膜功能、精子受精功能和精子成熟障碍指标的关系。结果:头部具有透明质酸结合位点的精子,顶体完整性[(95.4±3.9)%]、线粒体膜高电位率[(97.8±2.1)%]明显高于原始活动精子[(68.8±6.2)%和(72.8±7.4)%,P均<0.01];精子-透明质酸结合率与多项精液常规参数呈弱相关性(r=0.195~0.268,P均<0.05),与诱发顶体反应率和精子正常形态率呈正相关(r=0.666、0.417,P均<0.01),与精子核蛋白不成熟度、DNA碎片率和过量残留胞质率呈负相关(r=-0.266、-0.308、-0.218,P<0.01、0.01、0.05)。结论:头部存在透明质酸受体的精子,其质膜结构、受精潜能、成熟度俱佳;用于检测精子透明质酸受体的精子-透明质酸结合试验,是独立的精子多重质量评价新指标。更多还原

【Abstract】 Objective:To analyze the quality characteristics of human spermatozoa with hyaluronic acid(HA) receptors and search for a new indicator for the assessment of sperm quality.Methods:Using sperm-HA binding assay with HA-coated slides,we determined the binding rate of motile sperm with HA receptors and analyzed its correlation with routine semen parameters,sperm membrane function,sperm fertilizing function and diminished/arrested sperm maturation.Results:The motile sperm with HA binding sites in the acrosomal region showed significantly higher acrosomal integrity([95.4 ± 3.9]%) and mitochondrial membrane potential(MMP)([97.8 ±2.1]%) than those in the initial semen([68.8 ± 6.2]% and [72.8 ± 7.4]%)(P < 0.01).The sperm-HA binding scores were correlated mildly with many routine semen parameters(r = 0.195- 0.268,P < 0.05),positively with the acrosome reaction level after ionophore challenge(r = 0.666,P < 0.01) and normal sperm morphology(r = 0.417,P < 0.01),and negatively with sperm nucleoprotein immaturation(r =- 0.266,P < 0.01),DNA fragmentation(r =- 0.308,P < 0.01) and excessive residual cytoplasm(r =- 0.218,P < 0.05).Conclusion:Sperm with HA receptors in the acrosomal region exhibit significant advantages in plasma membrane structure,fertilizing potential and maturation.The sperm-HA binding assay,which is based on a relationship between sperm receptors for zona pellucida and HA,is likely to become a new independent indicator for assessing the multiple qualities of spermatozoa.更多还原