【摘要】 目的:国内外研究表明,新城疫病毒在体内外对多种肿瘤具有明显抑制作用。本研究旨在探讨新城疫病毒D90株体外对乳腺癌MCF-7细胞的作用及可能机制。方法:CCK-8法检测MCF-7细胞增殖抑制;光学显微镜及DAPI染色观察D90株对MCF-7细胞形态学影响;流式细胞术检测MCF-7细胞凋亡率;Western blot检测MCF-7细胞凋亡相关蛋白的表达情况。结果:D90株对MCF-7细胞具有显著抑制作用并呈浓度和时间依赖性;D90株能够诱导MCF-7细胞凋亡并调节凋亡相关蛋白的表达,包括上调Bax蛋白、下调Bcl-2蛋白,并促进Caspase-3和Caspase-9的裂解活化。结论:D90株能够调节凋亡相关蛋白的表达,并促进Caspase-3和Caspase-9的裂解活化,通过线粒体途径诱导MCF-7细胞凋亡并呈浓度和时间依赖性。更多还原

【Abstract】 Objective:Studies showed that Newcastle disease virus(NDV) had significant inhibitory action on many tumors in vitro and in vivo.This experiment was designed to investigate the effects and possible mechanism of NDV-D90 strain on breast cancer MCF-7 cells in vitro.Methods:Growth inhibition of MCF-7 cells was detected by CCK-8 method.The morphological changes of MCF-7 cells caused by D90 strain were observed by optical microscope and DAPI staining.MCF-7 cell apoptosis rate was detected by flow cytometry.The expression of apoptosis related proteins of MCF-7 cells was detected by Western blot.Results:NDV-D90 strains had inhibitory effect on MCF-7 cells in a concentration and time dependent manner;D90 strain induced apoptosis of MCF-7 cells and regulated the expression of apoptosis related proteins,including up-regulating Bax expression,down-regulating Bcl-2 protein,and promote the cleavage and activation of Caspase-3 and Caspase-9.Conclusion:D90 strain could regulate the expression of apoptosis related protein,promote the cleavage and activation of Caspase-3 and Caspase-9.It could induce apoptosis of MCF-7 cells through the mitochondrial pathway in a concentration and time dependent manner.更多还原