【摘要】 为研究转基因作物的土壤生态安全性,于根际箱中种植2组大豆(A组:转基因品系OE-8、OE-7、RNAi-3和受体南农88-1;B组:转基因品系Gaga1 17-4、Gaga1 21-8、Gaga1 57和受体N2899),采集大豆花期根际土壤,分析硫元素含量和芳基硫酸酯酶活性,并采用Biolog微平板法,研究相比于受体,转基因品系对土壤微生物群落功能多样性的影响。结果表明:OE-8和RNAi-3与其受体相比,根际土壤硫元素含量显著降低;2组组内对比结果显示,根际土样芳基硫酸酯酶活性差异均不显著。Biolog GN/GP/FF/ECO系统及多样性指数分析显示:A组中RNAi-3根际土壤微生物群落活性显著低于受体,微生物群落的丰富度、多样性和均度显著降低,OE-7根际土壤微生物群落的丰富度和均度显著降低;B组中Gaga1 57和Gaga1 17-4根际土壤微生物群落的丰富度、多样性和均度显著下降。更多还原

【Abstract】 Growing transgenic crops may have ecological consequences. In order to evaluate soil ecological risks of transgenic crops, a rhizo-box experiment was carried out to examine sulfur content, arylsulfatase activity, and microbial functional diversities in the rhizospheric soils grown with two groups of soybean:Group A(transgenic lines OE-8, OE-7, RNAi-3 and their recipient Nannong 88-1 as CK-1) and Group B(transgenic lines Gaga1 17-4, Gaga1 21-8, Gaga1 57 and their recipient N2899 as CK-2). At florescence stage, the content of sulfur in the rhizospheric soils of OE-8 and RNAi-3 was significantly lower than that of CK. Arylsulfatase activity had no significant difference between transgenic lines and their CKs. The Biolog analysis(GN/GP/FF/ECO systems)showed that the average well color developments(AWCDs)of rhizospheric soil of Line RNAi-3 in the Group A were significantly lower than the control, illustrating that planting Line RNAi-3 inhibited the physiology of soil Gram-negative(G-)bacteria, Gram-positive(G+)bacteria and fungi. However, in Group B the AWCDs values of Gaga1 21-8 significantly increased, implying that planting Line Gagal 21-8 stimulated the physiology of soil G+bacteria. Compared with the control, the richness, diversity and evenness of soil microbial community in Line RNAi-3 were all significantly lower; and the richness and evenness in Line OE-7 were also significantly declined. Significant reduction in the richness, diversity and evenness in both Line Gaga1 57 and Line Gaga1 17-4 was observed. It is concluded that growing transgenic soybeans has negative impacts on soil microbial activities and microbial functional diversity in rhizospheric soils.更多还原