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池塘养殖刺参病原菌塔式弧菌的分离与鉴定

Identification of Vibrio tubiashii isolated from diseased pond-cultured sea cucumbers( Apostichopus japonicus)

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【作者】 杨求华葛辉方旅平林琪何丽斌周宸

【Author】 YANG Qiuhua;GE Hui;FANG Lüping;LIN Qi;HE Libin;ZHOU Chen;Fisheries Research Institute of Fujian Province;Ocean and Earth Institute of Xiamen University;

【机构】 福建省水产研究所厦门大学海洋与地球学院

【摘要】 2013年春季,福建漳州地区池塘养殖刺参(Apostichopus japonicus)感染不明病原而患病。病参表现出排脏、身体萎缩、体表溃烂等症状,俗称为"腐皮综合征"。为确定引起该病的病原菌,从患病刺参病灶部位分离得到1株优势菌FJY001,经回接感染试验,证实所分离的细菌为刺参的病原菌。经形态、生理生化、16S rRNA序列分析和Biolog微生物自动鉴定系统等多项指标鉴定,确定该病原菌为塔式弧菌(Vibrio tubiashii)。

【Abstract】 In the spring of 2013,the pond-cultured sea cucumbers( Apostichopus japonicus) were infected by an unrestrained pathogen in Zhangzhou City of Fujian Province. The sea cucumbers with the disease tentatively termed as"skin ulcerative syndrome"showed symptoms such as viscera ejection,general atrophy and skin ulceration. To determine the bacterial pathogen that caused the disease,one strain( FJY001) was isolated from the lesions of diseased sea cucumbers. Pathogenicity of the strain was confirmed by artificial infection and the strain was identified as Vibrio tubiashii by indicators of morphological,physiological and biochemical characteristics as well as sequencing of 16 S rRNA and identification of Biolog microbiol system.

【关键词】 刺参病原菌塔式弧菌
【Key words】 Apostichopus japonicuspathogenVibrio tubiashii
【基金】 福建省科技厅公益类科研专项(2010R1002-3);福建省水产研究所科研发展基金资助项目(2014fjscq04)
  • 【文献出处】 南方水产科学 ,South China Fisheries Science , 编辑部邮箱 ,2014年04期
  • 【分类号】S947.9
  • 【被引频次】18
  • 【下载频次】219
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