【摘要】 建立了高效液相色谱-串联质谱法测定乳腺癌患者血清中多巴、酪氨酸、对羟苯基乳酸和酪胺的分析方法。在已优化的质谱条件下,待测物在6min内完成分离,且效果良好。结果表明,4种物质的检出限在0.000 5~0.20mg·L-1之间;定量限在0.001~0.40mg·L-1之间;线性相关系数在0.998 9~0.999 7之间,线性关系较好。血清中4种待测物在3个不同加标水平下的平均回收率在85.2%~114.0%之间,相对标准偏差在0.7%~8.5%之间。以上结果表明,该方法准确可靠,可用于实际样品的测定。更多还原

【Abstract】 A simple,selective,rapid and reliable method for the simultaneous determination of 3,4-dihydroxyphenylalanine(DOPA),tyrosine(Tyr),4-hydroxyphenyllactic acid(PHPLA)and 4-hydroxyphenethylamine(TA)in human serum by high-performance liquid chromatography(HPLC)coupled with tandem mass spectrometry(MS/MS)was proposed for the first time.Under optimized conditions,all compounds were resolved within 6min.The calibration curves were indicative of good linearity(correlation coefficients>0.998 9),and the limits of detections and quantifications for Tyr and its metabolites ranged from 0.000 5to 0.20mg·L-1 and 0.001 to 0.40mg·L-1,respectively.Recoveries of Tyr and its metabolites in breast cancer patient were in the range of 85.2%~114.0%,with RSDs of 0.7%~8.5%.The present method was successfully applied to the determination of DOPA,Tyr,PHPLA and TA in sera from breast cancer patients.更多还原