【摘要】 目的体外分离培养和鉴定小鼠脂肪源性干细胞,向成骨方向分化,为后续的实验选取合适的种子细胞奠定基础。方法从白色脂肪组织中分离培养脂肪源性干细胞,应用流式细胞仪分析其表面的标记。分别通过碱性磷酸酶、茜素红和油红-O染色方法染色方法鉴定向成骨和成脂方向分化。结果脂肪源性干细胞高表达表面标记CD29、CD44,并且能够向成骨和成脂方向分化。结论利用胶原酶消化法,在小鼠脂肪组织中成功分离、培养出脂肪源性干细胞,获得的细胞具有多向分化潜能,为后期组织工程技术修复骨组织,提供了坚实的实验基础。更多还原

【Abstract】 Objective Toisolate,cultureandidentifyadipose-derivedstemcells(ADSCs)frommouseanddifferentiate into bone so as to provide a theoretical basis for later experiments which are aim to select suitable seed cells for bone repair. Methods Adipose-derived stem cells were isolated and cultured from white adipose tissue, and surface antigens including CD44 were selected. CD29 and Sca-1 were analyzed by flow cytometry. Differentiation towards adipocyte and osteocyte were determined with different staining methods of alkaline phosphatase, Alizarin red stain and oil-O. Results ADSCs were positive for the pluripotent markers of CD29, CD44 and Sca-1, and able to finish osteogenic and adipogenic differentiation. Conclusions ADSCs can been succeeded to isolate and cultured through the method of collagenase from mouse adipose tissue. The obtained ADSCs could differentiate into diverse cell types. It provides a theoretical basis for later experiments which are aim to repair bone damage tissue by using tissue engineering.更多还原