【摘要】 目的探讨脂多糖(LPS)对呼吸道合胞病毒(RSV)感染小鼠的气道炎症及气道高反应性的影响及其机制。方法将6~8周龄雌性Balb/c小鼠分为4组:对照组、LPS组、RSV组及RSV+LPS组,于首次处理后第7天收取肺组织、肺泡灌洗液标本,QPCR检测肺组织病毒拷贝数,计数肺泡灌洗液(BALF)中细胞总数及分类计数,H&E染色观察肺部病理损伤,肺功能测定AHR,Western blot检测TRIF和MyD88蛋白表达,ELISA检测BALF中IFN-γ、KC、IL-1β、IL-6质量浓度。结果 RSV+LPS组病毒拷贝数与RSV组基本一致。RSV+LPS组BALF中细胞总数较LPS组、RSV组增加,且RSV组以淋巴细胞为主,RSV+LPS组以中性粒细胞为主。LPS组、RSV组、RSV+LPS组肺组织炎症及评分均较对照组明显增高。RSV+LPS组AHR较RSV组、LPS组增高。RSV+LPS组肺组织中TRIF表达较LPS组、RSV组增高,各组MyD88基本无变化。RSV组BALF中IFN-γ明显增高,RSV+LPS组KC明显增高,IL-1β、IL-6各组基本无变化。结论 LPS刺激可通过TRIF-KC途径加重RSV感染小鼠气道炎症及AHR。更多还原

【Abstract】 The present study was performed to investigate the effects of lipopolysaccharide(LPS) on airway inflammation and hyper-responsiveness induced by respiratory syncytial virus(RSV) infection and the underlying mechanism.Female Balb/c mice(6-8 weeks old) were divided into 4 groups:control,LPS group,RSV group,and RSV+LPS group.Seven days after the first treatment,viral copy number in lung was detected by Q-PCR;infiltration of inflammatory cells in bronchoalveolar lavage fluid(BALF),lung tissue damage as well as AHR were assessed;the expressions of TRIF and MyD88 in the lung tissue were measured by Western blotting,and the concentrations of IFN-γ,KC,IL-1β,and IL-6 in BALF were detected by ELISA.Result showed that viral copies of RSV in RSV+LPS group and RSV group were similar;the influx of leukocytes were remarkably higher in the RSV+LPS group than that of other groups,and the infiltrating cells were lymphocytes-dominant in the RSV group while neutrophilsdominant in the RSV+LPS group;lung tissue damage was significantly increased in LPS group,RSV group and RSV+LPS group,as compared to control;AHR in the RSV+LPS group was higher than that of LPS group and RSV group.The expression levels of TRIF in RSV+LPS group were higher than that in LPS group and RSV group;while levels of MyD88 showed no difference among the 4 groups.IFN-γ was markedly higher in the RSV group in contrast to other groups,while KC was significantly higher in the RSV+LPS group.There were no differences of IL-1β and IL-6 among the 4 groups.In conclusion,LPS could aggravate airway inflammation and AHR induced by RSV through the TRIF-KC signaling pathway.更多还原