【摘要】 目的探讨重组人白细胞介素-24(rhIL-24)联合顺铂(DDP)对人肺腺癌A549细胞的体外抑制效应及对A549细胞凋亡、细胞周期的影响。方法 rhIL-24、DDP及rhIL-24+DDP干预A549细胞,用CCK-8法检测A549细胞增殖抑制率;用流式细胞仪检测细胞凋亡及细胞周期的变化。结果 rhIL-24联合DDP作用24 h对人肺腺癌A549细胞增殖抑制率为32.8%,与单独DDP组比较有显著性差异(P<0.05);流式细胞仪检测rhIL-24联合DDP作用24 h对人肺腺癌A549细胞凋亡率为24.9%;细胞周期检测中rhIL-24作用A549细胞24 h后S期细胞增多。结论 rhIL-24能抑制A549细胞增殖,联合DDP组效果优于单独用药组,具有化疗增敏效应;rhIL-24能诱导A549细胞凋亡并能影响细胞周期。更多还原

【Abstract】 The purpose of this study is to investigate the inhibitory effects of recombinant human interleukin-24(rhIL-24) combined with Cisplatin(DDP) on human lung adenocarcinoma cell lines A549 in vitro, and to observe the changes in apoptosis and cell cycle. The cell proliferation was detected by CCK8 assay, and the changes in apoptosis and cell cycle of A549 was detected by flow cytometry after the treatment with rhIL-24 alone, DDP alone,and rhIL-24 combined with DDP respectively. We found that the treatment with rhIL-24 significantly inhibited the growth of human lung adenocarcinoma cell lines A549 in vitro. While treatment of rhIL-24(160 ng/ml) plus DDP of24 h resulted in a 32.8% growth inhibition on A549, which showed a significant difference to DDP alone group; and the apoptosis rates of A549 was 24.9% after treatment of rhIL-24(160 ng/ml) plus DDP for 24 h. Furthermore, cell cycle detection indicated that the percentage of A549 in S phases increased in rhIL-24 alone group. In conclusion,the treatment with rhIL-24 led to the inhibition of A549 growth, while rhIL-24 plus DDP has a better anti-lung adenocarcinoma effect than rhIL-24 alone, and could enhance the effect of chemotherapy. rhIL-24 also can induce alteration of apoptosis and cell-cycle in human lung adenocarcinoma A549.更多还原