【摘要】 目的:观察透明质酸(hyaluronic acid,HA)对大鼠自体颗粒脂肪移植物存活的影响。方法:18只Wistar大鼠(雄雌不限,体重220±5g)背部皮下分别注射自体颗粒脂肪0.3ml、透明质酸0.3ml和自体颗粒脂肪与透明质酸混合物0.3ml(颗粒脂肪0.15ml+透明质酸0.15ml),并记为Fat组、HA组、Fat+HA组,分别于第30天、第60天、第90天,检测移植物的体积并计算成活率;HE染色分析脂肪细胞的形态和移植物的炎症反应;免疫组织化学染色检测CD31表达,计数血管情况。结果:1注射Fat、HA、Fat+HA(1:1)0.3ml后,大鼠背部形成三个长条形的局部隆起,注射第15天HA组局部隆起消失,注射后第30天,第60天,第90天Fat+HA组移植物的成活率比Fat组高,具有统计学意义(P<0.05);2局部取材切片(HE染色)可观察到炎症反应程度逐渐减轻,Fat+HA组诱发组织炎症反应较Fat组为轻;3注射后第30天,第60天,第90天时CD31标记组织内血管计数Fat-HA组多于Fat组,具有统计学意义(P<0.05)。结论:透明质酸混合自体颗粒脂肪注射可以提高颗粒脂肪的成活率、促进移植物的再血管化、维持脂肪移植物的体积。更多还原

【Abstract】 Objective Autologous fat transplantation is a common technique for soft tissue augmentation in aesthetic and reconstructive surgery; however, the degree of fat graft take can be unpredictable. Hyaluronic acid has been shown to be a promising cell carrier in adipose tissue engineering. Objectives To investigate the effect of a hyaluronic acid on autologous fat graft survival,angiogenesis, and volume maintenance in rats. Methods Fat was harvested from the groins of 18 rats, processed, and injected beneath the animals’ dorsums to form three grafts: one containing fat alone and one containing hyaluronic acid alone,one containing fat and hyaluronic acid in a 1:1 mix.The grafts were removed at 30 th day, 60 th day, 90 th day,to measure fat graft volume and calculate the survival rate of the graft,as well as to observe the color, shape and volume of the grafts..Histological studies were performed after sacrifice to evaluate fat necrosis and blood vessel density.Results All grafts were clinically viable. HA aft disappeared at 15 th day after injection.The survival rate of Fat-HA graft of 30 th day,60 th day and 90 th day were significantly higher than Fat graft(P <0.05). At30 th day, 60 th day and 90 th days after injection, blood vessel density in Fat-HA grafts was significantly greater than those in the grafts containing fat alone(P <0.05). Conclusion When mixed with fat, hyaluronic acid can improve early fat graft survival and may enhance vascularity and prolong volume maintenance.更多还原