【摘要】 目的:利用豚鼠脊髓匀浆建立Wistar大鼠实验性自身免疫性脑脊髓炎(EAE)的动物模型,为中枢神经系统脱髓鞘性疾病的研究提供实验依据。方法:以豚鼠全脊髓匀浆(GPSCH)与完全弗氏佐剂(CFA)的混合乳剂一次性给予大鼠足垫皮下注射,同时大鼠足背注射百日咳杆菌原液,建立EAE模型。结果:在免疫后12天开始出现肢体瘫痪,双后肢拖动等EAE临床症状。光镜下可见脑和脊髓组织中有大量炎性细胞浸润,炎性细胞聚集在血管周围呈典型的"袖套"样改变。结论:利用豚鼠脊髓匀浆制备Wistar大鼠EAE模型方法简单,稳定、可靠、发病率高。更多还原

【Abstract】 Objective:To induce experimental autoimmune encephalomyelitis(EAE) in Wistar rat treated with guinea pig spinal cord homogenate(GPSCH),and to provide the experimental value for the demyelinating disease of the central nervous system(CNS).Method:Wistar rats were immunized with both GPSCH and CFA and Bordetelle pertussis.,the pathological changes of EAE were studied with the aid of light microscopy.Results:The animal got the clinical symptom of EAE; the characteristic light microscopical findings are:perivascular inflammatory infiltration,inflammatory cuffs of small blood vessels.Conclusion:The method of establishing the EAE model is stable,simple and of high incidence.更多还原