【摘要】 目的评价突触后致密物（PSD）95多肽疫苗对神经病理性痛大鼠的镇痛效应。方法成年雌性SD大鼠,制备保留性神经损伤（SNI）模型,7d后选取SNI模型制备成功的大鼠30只,随机分为5组（n=6）:NP1组、NP2组、磷酸盐缓冲液（PBS）组、匙孔虫戚血蓝蛋白（KLH）组及PSD-95组。PSD-95组沿背部两侧皮下多点注射PSD-95多肽疫苗50μl（含PSD-95 100μg）3次,每隔2周注射1次;PBS组和KLH组分别给予PBS 50μl和KLH 100μg,均用弗氏佐剂稀释至100μl。分别于制备SNI前1d、第1次免疫前1d及第3次免疫后14d（T0-2）时测定机械痛阈;于T1时处死NP2组大鼠,于T2时处死其余各组大鼠,取L_3⁵脊髓背角组织,采用Elisa法测定脊髓背角脑源性神经营养因子（BDNF）表达水平。结果与T0时比较,T1时各组机械痛阈均降低（P<0.05）。与T1时比较,PSD-95组T2时机械痛阈升高,BDNF表达下调（P<0.05）;PBS组和KLH组T2时指标差异无统计学意义（P>0.05）。与NP1组比较,PSD-95组T2时机械痛阈升高,BDNF表达下调（P<0.05）;PBS组和KLH组T2时指标差异无统计学意义（P>0.05）。结论 PSD-95多肽疫苗对神经病理性痛大鼠具有镇痛作用,其机制可能与下调脊髓BDNF表达有关。更多还原

【Abstract】 Objective To investigate the analgesic effect of postsynaptic density(PSD)-95 peptide vaccine on neuropathic pain(NP) in rats.Methods Adult female SD rats were selected to produce retained nerve injury(SNI) model.Seven days after successful SNI surgery,30 rats were randomly divided into 5 groups(n =6);NP1 group,NP2 group,PBS group,keyhole limpet hemocyanin(KLH) group and PSD-95 group.Rats in PSD-95 group were given mulli-point injection of PSD-95 peptide vaccine on the back subcutaneously,on both sides 3times at 2 weeks interval,and those in PBS and KLH groups were given PBS or KLH.The mechanical pain threshold was determined one day before the preparation of SNI(underlying value),1 day before the first immunization,14 days after the third immunization(TO-2).Rats of NP2 group were killed at T1,and the left rats were killed at T2 to determine the expression of brain-derived neurotrophic factor(BDNF) in the spinal cord lumbar enlargement.Results Compared with TO,the mechanical pain threshold of each group at Tl was decreased(P < 0.05).Compared with Tl,the mechanical pain threshold in PSD-95 group at T2 was increased(P <0.05).And the expression of BDNF was downregulated(P < 0.05).There was no significant difference in various indicators between PBS group and KLH group at T2(P > 0.05).Compared with NPl group,the mechanical pain threshold in PSD-95 group at T2 was increased(P < 0.05).And the expression of BDNF was downregulated(P < 0.05).There was no significant difference in various indicators between PBS group and KLH group at T2(P > 0.05).Conclusion PSD-95 peptide vaccine has analgesic effects on neuropathic pain in rats,which may be related to the downregulation of the expression of spinal BDNF.更多还原