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豫产道地金银花HPLC指纹图谱研究
Study on HPLC Fingerprint of Lonicera Japonica Thunb.from Henan Genuine
【摘要】 目的:用高效液相色谱法建立豫产道地金银花指纹图谱分析方法。方法:采用ODS色谱柱,流动相为乙腈、混合酸梯度洗脱,检测波长为243 nm。结果:标示出金银花19个共有色谱峰。结论:金银花中各成分均得到很好分离,可作为豫产道地金银花的专属指纹图谱。
【Abstract】 Objective:To establish the fingerprint menthod of Lonicera japonica Thunb.from Henan genniu.Method:The HPLC method was used.The chromatography conditions were ODS column,acetonitrile as the mobile phase A,a mixed acid solution as the mobile phase B,gradient elution.Detectice wavelength was 243 nm.Result:The result shouwed that 19 peaks were common.Conclusion:The various components of Lonicera japonica Thunb.from Henan genuine were well isolated,which can serve as the specific fingerprint chromatography.
【关键词】 河南;
道地;
金银花;
指纹图谱;
高效液相色谱法;
【Key words】 Henan; genuine; Lonicera japonica Thunb.; fingerprint; HPLC;
【Key words】 Henan; genuine; Lonicera japonica Thunb.; fingerprint; HPLC;
【基金】 河南省科技攻关资助项目(0423030500-4)
- 【文献出处】 辽宁中医药大学学报 ,Journal of Liaoning University of Traditional Chinese Medicine , 编辑部邮箱 ,2014年09期
- 【分类号】R284.1;O657.72
- 【被引频次】3
- 【下载频次】160