【摘要】 目的观察Rnd1在难治性癫痫颞叶脑组织中的表达情况,探讨Rnd1在难治性癫痫发病的作用。方法收40例难治性癫痫患者脑组织颞叶(实验组)以及10例正常颞叶脑组织(对照组),用免疫组化、免疫荧光、Western-blot方法检测患者颞叶脑组织Rnd1的表达,并比较实验组与对照组颞叶脑组织中Rnd1的表达情况。结果免疫组化显示实验组Rnd1的平均灰度(127.62±6.23)与对照组(171.79±3.84)相比差异有统计学意义(t=29.86,p<0.01);实验组Rnd1的阳性细胞数(30.20±3.42)与对照组(11.18±0.67)相比差异有统计学意义(t=21.36,p<0.01)。免疫荧光显示Rnd1的阳性表达区域与特异性神经无性烯醇化酶(NSE)的分布基本重叠。结论 Rnd1主要表达于神经元,可能参与了癫痫的形成并在难治性癫痫形成的过程中发挥了重要作用。更多还原

【Abstract】 Objective To investigate the expression of Rnd1 in the brain tissue of intractable epilepsy and explore the pathogenic of intractable epilepsy.Methods To investigate the role of Rnd1 protein in pathophysiology ofIE,we studied the expression of Rnd1protein in surgical samples of the temporal neocortex of patients with IE and the agematched controls samples.Results Immunohistochemistry showed the mean gray level of Rnd1 in the temporal lobe of the patient( 127.62 ±6.23) significanfly lower than that of the controls( 171.79±3.84),t = 29.86,p<0.01); the cell count of Rnd1 positive cells in the temporal lobe of the patient( 30.20±3.42) was significantly higher( t = 21.36, p<0.01) than that of the controls( 11. 18 ± 0. 67). Immunofluorescence showed the positive expression of Rnd1 distribution overlapping the distribution of neuron-specific enolase( NSE).Conclusion Rnd1 up-regulate in the brain tissue of patients with intractable epilepsy,it suggests Rnd1 may involve in the formation of intractable epilepsy pathogenic.更多还原