【摘要】 目的:探讨人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)发育过程中,全反式维A酸(ATRA)和(或)三氧化二砷(As2O3)对HoxB8 mRNA/蛋白表达的影响,为ATRA和As2O3治疗提供依据。方法:12例脐带血样本采自胎盘段脐带血,在人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)增殖分化过程中,加入ATRA 10 nmol/L和(或)As2O310 nmol/L持续干扰培养的细胞。采用real-time PCR和Western Blotting方法分别测定HoxB8 mRNA/蛋白的表达水平。结果:人类造血干细胞向粒系祖细胞增殖分化过程中,对照组、ATRA组、As2O3组及ATRA+As2O3组的HoxB8 mRNA/蛋白第3 d开始表达,第7 d表达较强烈,第12 d表达减弱;在real-time PCR方法中,与对照组比较,ATRA组、As2O3组及ATRA+As2O3组HoxB8 mRNA的表达上调(P<0.05)。在Western Blotting方法中,与对照组比较,ATRA组、As2O3组及ATRA+As2O3组HoxB8蛋白的表达上调(P<0.05)。结论:在人脐血造血干细胞(HSC)向粒系祖细胞(CFU-G)发育过程中,HoxB8基因与粒系造血密切相关。ATRA、As2O3及ATRA+As2O3能显著上调HoxB8 mRNA及蛋白的表达。更多还原

【Abstract】 Objective: To investigate the effect of all-trans retinoic acid(ATRA)and arsenic trioxide(As2O3)on homeoboxB8(HoxB8)mRNA and protein expressions. Methods: Twelve cord blood samples were collected from the fetal placenta umbilical vein and cultured in vitro. The proliferation and differentiation of cord blood HSCs into CFU-G was continuously disrupted with 10 nmol/L of ATRA and/or 10 nmol/L of As2O3. The expression of HoxB8 mRNA and protein were detected by quantitative real-time polymerase chain reaction(qRT-PCR) and Western Blotting, respectively. Results: HoxB8 mRNA/protein expression was detectable on the 3rd day, reached its highest level on the 7th day and decreased on the 12 th day. HoxB8 mRNA/protein expression in ATRA,As2O3,and ATRA+As2O3groups was upregulated compared with those in control group(P < 0.05). Conclusion:There is positive relationship between HoxB8 gene and granulocyte progenitor hematopoiesis. ATRA/As2O3up-regulate the expression of HoxB8 mRNA/protein, and treatment of leukemia with ATRA/As2O3 may be through the regulation of Hox gene expression.更多还原