【摘要】 目的:证实α-Actinin 2与小电导钙激活钾通道(Small Conductance Ca²⁺-activated K⁺Channels,SK)的共同表达增加SK2在HEK293细胞膜上的表达。方法:HEK293细胞用RPMI 1640培养基培养。将HEK293细胞分为两组,对照组细胞转染pIRES—SK2质粒,实验组细胞共转染pIRES—SK2和pcDNA3—α-actinin2质粒。免疫荧光共聚焦屈微镜和蛋白印迹技术检测SK2通道蛋白在HEK293细胞膜上的表达。结果:免疫荧光共聚焦显微镜检测到转染pIRES—SK2质粒的HEK293细胞膜上有SK2通道蛋白的表达。蛋白印迹技术显示实验组蛋白条带亮度明显高于对照组。结论:α—Actinin 2能增加SK2在HEK293细胞膜上的表达。更多还原

【Abstract】 Objective:To confirm whether the protein expression of SK2 channel in HEK293 cell membrane is increased by the co-expression of a-Actinin2.Methods:HEK293 cells were cultured and randomly divided into control group,transfected with plasmid pIRES-SK2,and experimental group,transfected with plasmid pIRES-SK2plus pcDNA3-a-Actinin2.Immunofluorescence confocal microscopy and western-blotting were used to test the membrane protein expression of SK2 channel in HEK293 cell.Results:SK2 channel protein was detected in the membrane of HEK293 by immunofluorescence confocal microscopy,and he protein expression level in the experimental group was much higher than that in the control group.Conclusion:a-Actinin2 can increase SK2 channel membrane protein expression in HEK293 cell.更多还原