【摘要】 对脱细胞真皮基质(acellular dermal matrix,ADM)囊袋植入兔角膜后的生物相容性、生物学转归及组织结构改变进行生物力学研究,以探讨其作为组织工程角膜支架材料的可行性.对ADM进行了系统的组织微观结构观察和植入性实验研究,组织结构观察包括HE染色、胶原染色、免疫荧光染色和透射电镜观察.在植入实验中,将ADM按不同预处理方法分为无干预组、脱水组和复水组,将3组ADM囊袋植入兔角膜,观察其术后不同时期在体和离体的生物相容性和组织学特性.结果发现:ADM由横纵交替、排列疏松的Ⅰ和Ⅲ型胶原纤维束构成,未见细胞结构;囊袋植入角膜后有不同程度的炎症反应,部分植片透明;组织学观察显示3组ADM植入角膜后均有细胞长入,并进行了胶原纤维重塑.研究工作表明,经适当预处理且结构致密的脱细胞真皮基质植入后可以长期稳定存留于角膜内,是一种比较理想的组织工程角膜支架.更多还原

【Abstract】 The biocompatibility, biological changes and tissue structure of acellular dermal matrix(ADM) in lamellar corneal transplantation(LCT) as a substitute are experimentally investigated in order to evaluate its possibility as a supporting material in corneal tissue engineering. The microstructure of ADM was observed with light microscope(LM),fluorescence microscope, polarizing microscope, and transmission electron microscope(TEM). In the implantation experiment, the ADM was processed with 3 different methods and implanted into rabbit corneal stroma. The ocular surface condition was observed with slit lamp microscope(SLM) postoperatively. The rabbits were sacrificed and morphology structures of the corneal grafts were studied by LM and polarizing microscope at 1 week, 4 weeks, 12 weeks and 24 weeks after transplantation. It was found that the ADM were composed mainly of Type-I and III collagens organized loosely and disorderly. No cellular structure was observed. There were inflammatory responses after implantation. Some implants turned to be transparent. Histological study evidenced both the growth of cells into the implant and the reconstruction collagen fiber. The results showed that the ADM with compact structure and proper pretreatment could stably exist in corneal stroma after implantation and, therefore, could be used as a potential scaffold in corneal tissue engineering.更多还原