【摘要】 目的观察在2型糖尿病(T2DM)大鼠模型形成过程中ghrelin和下丘脑神经肽Y(NPY)的变化,探讨ghrelin及NPY在T2DM大鼠发病过程中的作用。方法选择SD大鼠60只,分为对照组15只、高脂饮食诱导肥胖(DIO)4周组(DIO 4周组)15只(高脂饮食喂养4周)、DIO 8周组15只(高脂饮食喂养8周)和T2DM组15只。以DIO结合小剂量链脲佐菌素(15mg/kg)法建立T2DM大鼠模型。应用酶联免疫吸附法检测大鼠空腹血浆及胃组织匀浆ghrelin水平;RT-PCR法检测下丘脑NPY mRNA的表达变化。结果与对照组比较,DIO4周组、DIO8周组和T2DM组大鼠血浆和胃组织ghrelin水平明显降低(P<0.01),NPY mRNA表达明显升高[(1.18±0.14)、(1.54±0.09)和(1.82±0.12)vs(0.88±0.17),P<0.01]。NPY mRNA与ghrelin水平呈负相关(r=-0.989,P<0.01)。结论 ghrelin可能通过刺激下丘脑NPY的合成和分泌,共同参与T2DM的形成。更多还原

【Abstract】 Objective To study the role of ghrelin and hypothalamic neuropeptide Y(NPY)in a rat model of type 2diabetes mellitus(T2DM)by observing their pathological changes.Methods Sixty SD rats were randomly divided into control group,4-week high fat diet-induced obesity(DIO4)group,8-week high fat diet-induced obesity(DIO8)group and T2 DM group(15in each group).The animals in DIO4 and DIO8groups were fed with high fat diet for 4and 8weeks,respectively.A rat T2 DM model was established by inducing obesity with high fat diet and low streptozotocin dose(15mg/kg).Serum levels of fasting ghrecin and gastric ghrelin were measured by ELISA and expression of NPY mRNA in hypothalamus was detected by RT-PCR.Results The serum gastric ghrelin level was significantly lower whereas the NPY mRNA expression level was significantly higher in DIO4 group,DIO8group and T2 DM group than in control group(1.18±0.14 vs0.88±0.17,1.54±0.09 vs 0.88±0.17,1.82±0.12 vs 0.88±0.17,P<0.01).The NPY mRNA expression level was negatively related with the serum ghrelin level(r=-0.989,P<0.01).Conclusion Ghrelin is involved in the pathogenesis of T2 DM by stimulating the synthesis and secretion of NPY.更多还原