【摘要】 目的:探讨RNA干扰技术沉默STAT3基因调控腺样囊性癌细胞生长的实验研究。方法:人腺样囊性癌生长细胞株ACC 2常规培养,分别采用A组为空白转染组,B组转染阴性对照siRNA(NC),C组转染特异性的siRNA(siRNA组)转染进ACC2,采用qRT PCR和Western blot法检测转染后STAT3 mRNA和蛋白表达水平的变化。MTT检测转染后腺样囊性癌细胞增殖。结果:ACC2细胞转染STAT3 siRNA 48 h后,STAT3 mRNA和蛋白的表达均受到明显抑制(P<0.05)。ACC 2生长72 h细胞转染后细胞增殖受到抑制,较对照组有显著性差异(P<0.05)。结论:STAT3基因有可能成为腺样囊性癌细胞生长基因治疗中重要的靶基因。更多还原

【Abstract】 Objectives;To explore the effect of downregulation STAT3 expression on the growth of adenoid cystic carcinoma(ACC).Method:STAT3 siRNA were designed and chemical synthesized,then transfected into the human adenoid cystic carcinoma cell line,qRT- PCR and Western blot assay were used to detect STAT3 mRNA and protein expression level.MTT were used to detect proliferation in ACC2 cell.Result:STAT3 mRNA and protein expression in ACC2 cell transfected with siRNA STAT3 were inhibited significandy(P<0.05) compared with the NC control group.Proliferation in ACC2 cell was inhibited significantly,and apoptosis was induced significantly compared with the NC control group(P<0.05).Conclusion;STAT3 gene may be an important target gene in gene therapy to adenoid cystic carcinoma cancer.更多还原