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增殖的胰腺β细胞可上调肿瘤干细胞标志物ALDH

Proliferating Pancreatic Beta Cells Upregulate Tumor Stem Cell Marker ALDH

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【作者】 刘应兰杨静曹仁贤姜孝新

【Author】 LIU Yinglan;YANG Jing;CAO Renxian;JIANG Xiaoxin;Department of Endocrinology,the First Affiliated Hospital of University of South China;Department of Clinical Laboratory, the First Affiliated Hospital of University of South China;

【机构】 南华大学附属第一医院内分泌科南华大学附属第一医院检验科

【摘要】 目的探讨肿瘤干细胞标志物乙醛脱氢酶(ALDH)在小鼠胰腺β细胞增殖模型中的表达情况。方法通过胰腺部分切除术构建小鼠胰腺β细胞增殖的模型,采用流式细胞术和免疫荧光检测其胰腺β细胞中ALDH的表达,采用Western blot检测细胞周期相关蛋白Cyclin D1、CDK2及CDK4的表达。结果胰腺部分切除术后2周的胰岛细胞中aldefluor+细胞明显增多,Ki-67+β细胞比例明显升高,且aldefluor+细胞Cyclin D1、Cyclin D2和CDK4蛋白表达水平均明显高于aldefluor-细胞。胰腺部分切除术后小鼠胰腺中的ALDH+细胞绝大部分均为β细胞。结论胰腺部分切除术诱导的增殖胰岛β细胞具有较高的ALDH活性,aldefluor荧光标记可能作为筛选增殖β细胞的标记物。

【Abstract】 Objective To investigate the expression of aldehyde dehydrogenase(ALDH) in the beta-cell proliferation model. Methods The presence of aldefluor + beta cells was detected by flow cytometry(FCM) and immunofluorescence in partial pancreatectomy(PPX) model. The expression of cell-cycle activators CDK2, Cyclin D1 and CDK4 were detected by Western blot. Results The aldefluor+ beta cells are essentially all positive for Ki-67 in PPX model, and expressed high levels of cell-cycle activators Cyclin D1, Cyclin D2 and CDK4. Conclusion Our data thus revealed that the proliferating beta cells induced by partial pancreatectomy showed a high ALDH activity. Moreover, our data also suggest that aldefluor lineage-tracing might be a marker for screening proliferating beta cells.

【基金】 国家自然基金资助项目(81172542)
  • 【文献出处】 肿瘤药学 ,Anti-tumor Pharmacy , 编辑部邮箱 ,2014年06期
  • 【分类号】R730.2
  • 【被引频次】1
  • 【下载频次】70
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