【摘要】 目的探讨肿瘤干细胞标志物乙醛脱氢酶(ALDH)在小鼠胰腺β细胞增殖模型中的表达情况。方法通过胰腺部分切除术构建小鼠胰腺β细胞增殖的模型,采用流式细胞术和免疫荧光检测其胰腺β细胞中ALDH的表达,采用Western blot检测细胞周期相关蛋白Cyclin D1、CDK2及CDK4的表达。结果胰腺部分切除术后2周的胰岛细胞中aldefluor+细胞明显增多,Ki-67+β细胞比例明显升高,且aldefluor+细胞Cyclin D1、Cyclin D2和CDK4蛋白表达水平均明显高于aldefluor-细胞。胰腺部分切除术后小鼠胰腺中的ALDH+细胞绝大部分均为β细胞。结论胰腺部分切除术诱导的增殖胰岛β细胞具有较高的ALDH活性,aldefluor荧光标记可能作为筛选增殖β细胞的标记物。更多还原

【Abstract】 Objective To investigate the expression of aldehyde dehydrogenase(ALDH) in the beta-cell proliferation model. Methods The presence of aldefluor + beta cells was detected by flow cytometry(FCM) and immunofluorescence in partial pancreatectomy(PPX) model. The expression of cell-cycle activators CDK2, Cyclin D1 and CDK4 were detected by Western blot. Results The aldefluor+ beta cells are essentially all positive for Ki-67 in PPX model, and expressed high levels of cell-cycle activators Cyclin D1, Cyclin D2 and CDK4. Conclusion Our data thus revealed that the proliferating beta cells induced by partial pancreatectomy showed a high ALDH activity. Moreover, our data also suggest that aldefluor lineage-tracing might be a marker for screening proliferating beta cells.更多还原