【摘要】 目的 观察Krüppel样转录因子KLF2在内毒素诱导大鼠急性肺损伤(ALI)时的动态表达,分析两者与急性肺损伤的相关性。方法将100只SD大鼠随机分为正常对照组和模型组,模型组进一步随机分成三组,模型组采用尾静脉注射脂多糖(LPS,5mg/kg)复制ALI模型,分别于尾静脉注射2h、4h、24h后采血及肺组织。观察各组病理表现、RT-PCR检测KLF2mRNA在血清中及肺组织的表达,分析KLF2在急性肺损伤中的动态表达,采用SPSS17.0软件进行统计学分析。结果病理检查显示造模后4h肺损伤最为明显,KLF2 mRNA在正常大鼠肺组织及血清中均有明显表达,模型组各组KLF2的表达较对照组显著减弱(P<0.01),且KLF2 mRNA在2h表达明显低于4h组和24h组(P<0.01)。结论KLF2在大鼠急性肺损伤表达变化早于病理变化,KLF2可作为急性肺损伤早期诊断的分子标志物。更多还原

【Abstract】 Objective To research the dynamic expression of KLF2 in the acute lung injury(ALI) rats induced by lipopolysaccharide(LPS),and analysis the correlation of KLF2 in ALI.Methods A total of 100 SD rats were randomly divided into 2 groups of normal control group and LPS treated group,then the latter group were randomly divided into 3 groups,which respectively taken serum and lung tissue samples 2h,4h and 24 h after molding.The ALI model was induced by injecting 5mg/kg LPS in tail vein.To observe pathological manifestations of each group,detect KLF2 mRNA expression in serum and lung tissue by RTPCR,and analysis of dynamic expression,While using SPSS17.0 software for statistical analysis.Results The pathology shows most obvious damage of lung tissue is 4 hours after modeling,the expression of KLF2 mRNA in the lung tissue and serum of control group were clear,and significantly reduced in LPS treated group compared to control group(P<0.01),the expression of KLF2 in LPS treated group of 2hours was lower than groups of 4 hours and 24 hours(P<0.01).Conclusion The expression changes of KLF2 mRNA was earlier than the pathological changes in acute lung injury,which can be used as a predictive diagnostics candidate molecular markers of ALI.更多还原