【摘要】 采用不同浓度NaCl溶液(0,50,150,250 mmol/L)处理萌发的垂穗披碱草幼苗,通过检测垂穗披碱草在盐胁迫逆境下生理生化的变化,并与老芒麦进行比较,研究其幼苗对盐胁迫的反馈机制,为垂穗披碱草在盐碱地的建植和恢复改良提供参考.结果表明:垂穗披碱草叶片可溶性糖质量分数和过氧化氢酶活性变化一致,随着盐浓度的增加呈现先上升后下降的趋势,50 mmol/LNaCl是其幼苗可溶性糖质量分数和过氧化氢酶活性的一个转折临界点;相对含水量、脯氨酸质量分数和超氧化物歧化酶活性变化一致,随着盐浓度的增加呈现上升趋势,分别在150,250 mmol/L盐浓度胁迫下,垂穗披碱草幼苗相对含水量、脯氨酸质量分数和超氧化物歧化酶活性显著高于其他材料的.方差分析结果表明盐胁迫对垂穗披碱草、老芒麦的生理指标影响显著.经相关分析筛选出对抗盐性影响显著的指标为相对含水量、可溶性糖质量分数、脯氨酸质量分数、超氧化物歧化酶、过氧化氢酶活性.经隶属函数法计算得出,垂穗披碱草具有一定的抗盐性,而且较老芒麦的强.更多还原

【Abstract】 Compared with those of Elymus sibiricus, the growth parameters of Elymus nutans at the early seedling stage were measured under four NaCl solutions with different concentrations(0, 50, 150, 250 mmol/L) and salt treatment was performed to explore and utilize the germplasm with high salt-tolerance and to provide physiological indicators for identifying salt-tolerance. It was found that the soluble sugar content and the activities of CAT were increasing at first and then declining with prolonged salt stress; a critical turning point occurred under the salt stress at 150 mmol/L, the relative water content, the proline content and the activities of SOD increased continually with NaCl stress concentration being increased, and accessions were higher than the others when the salt concentration was at 150 mmol/L and 250 mmol/L. Statistics analysis showed that the effects were different for E. nutans and E. sibiricus under salt stress. Correlation analysis indicated that the indices which had significant impact on salt resistance were the relative water content, soluble sugar content, proline content, and the activities of SOD and CAT. Calculated by the membership function, the salt-resistant ability of E. nutans was better than that of E. sibiricus.更多还原