【摘要】 目的:探讨肿瘤坏死因子样弱凋亡诱导因子(tumor necrosis factor-like weak inducer of apoptosis,TWEAK)对体外培养的大鼠关节软骨细胞产生趋化因子的影响,初步了解TWEAK在OA发病机制中的作用。方法:体外培养大鼠关节软骨细胞,实验组细胞用250 ng/mL TWEAK诱导,对照组则不作此处理,24、48、72 h后检测两组细胞培养液中趋化因子白细胞介素-8(interleukin-8,IL-8)、单核细胞趋化蛋白-1(monocyte chemotactic protein 1,MCP-1)、受激活调节正常T细胞表达和分泌因子(regulated upon activation normal T cell expressed and secreted factor,RANTES)和巨噬细胞炎性蛋白-2(macrophage inflammatory protein 2,MIP-2)的含量。结果:TWEAK诱导24、48、72 h后,实验组大鼠关节软骨细胞上清液中RANTES含量均明显高于相应对照组(P<0.05),且RANTES的含量与TWEAK作用时间呈正相关(P<0.05);实验组IL-8和MCP-1的含量在TWEAK诱导48、72 h后均明显高于相应对照组(P<0.05),但与TWEAK作用时间无相关性(P>0.05);MIP-2的浓度在TWEAK诱导48、72 h后均明显低于相应对照组(均P<0.05),且其含量的降低与TWEAK作用时间呈正相关(P<0.05)。结论:TWEAK能诱导体外培养的大鼠关节软骨细胞产生趋化因子(IL-8、MCP-1、RANTES),以此参与OA的发生。更多还原

【Abstract】 Objective:To explore the effect of tumor necrosis factor-like weak inducer of apoptosis( TWEAK) on generating chemokines in rat articular chondrocytes cultured in vitro,and to understand its role in the pathogenesis of OA. Methods:Rat articular chondrocytes were cultured in vitro. The cells of experimental group were induced by 250 ng /mL TWEAK,while the cells of the control group were not. Levels of chemokines interleukin-8( IL-8),monocyte chemotactic protein 1( MCP-1),regulated upon activation normal T cell expressed and secreted factor( RANTES),macrophage inflammatory protein 2( MIP-2) in the nutrient solution of the two groups were detected at 24,48 and 72 h. Results: After the induction of TWEAK,the levels of RANTES in the supernatant of rat articular chondrocytes at 24,48 and 72 h were all significantly higher than those in the control groups,respectively( all P < 0. 05),and the contents of RANTES were positively correlated with the treatment time of TWEAK( P < 0.05). The IL-8 and MCP-1 levels in the supernatant of rat articular chondrocytes at 48 and 72 h were significantly higher than those in the control groups( both P < 0. 05),but they had no correlations with the treatment time of TWEAK( P > 0. 05). The MIP-2 levels of the rat articular chondrocytes supernatant at 48 and 72 h were significantly lower than those in the control groups(P <0. 05),and the contents of MIP2 were positively correlated with the treatment time of TWEAK(P <0. 05). Conclusions:TWEAK can induce rat chondrocytes in vitro to generate chemokines( IL-8,MCP-1,RANTES),so that it is involved in the occurance of OA.更多还原