【摘要】 目的探讨实时荧光定量PCR法检测HBV-DNA结果与临床疾病状态关系。方法采用实时荧光定量PCR技术对518例乙肝患者血清进行HBV-DNA测定,同时进行肝功能指标及乙肝两对半的检测。结果 518例乙肝病毒感染者血清h BV-d NA阳性率为67.8%(351/518),慢性乙肝阳性率为69.4%(283/408),肝硬化阳性率为67.9%(57/84),原发性肝癌阳性率为60%(6/10);162例HBs Ag、HBe Ag、抗-HBc阳性标本,实时荧光定量PCR检测HBV-DNA有152例为阳性,阳性率为93.8%,平均HBVDNA浓度为2.84×107;在261例HBs Ag、抗HBe、抗-HBc阳性的标本中,实时荧光定量PCR检测HBV-DNA阳性128例,阳性率为64.3%,平均HBV-DNA浓度为3.29×104。结论实时荧光定量PCR法检测HBV-DNA更能真实反映乙肝病毒在体内的复制和感染程度。更多还原

【Abstract】 Objective To investigate the relationship between the results of HBV-DNA real-time fl uorescent quantitative PCR method and the clinical status. Methods 518 cases of hepatitis B patients, serum HBV-DNA was determined by real-time fl uorescence quantitative PCR detectiontechnology, at the same time the index of liver function and hepatitis B two half and half. Results the positive rate of serum HBV-DNA in 518 patients with hepatitis B virus infection was 67.8%(351/518), the positive rate of chronic hepatitis B was 69.4%(283/408), the positive rate was 67.9%(57/84) in liver cirrhosis,primary hepatocellular carcinoma is a positive rate of 60%(6/10); 162 cases of HBs Ag,HBe Ag,anti –HBc positive specimens, real-time fl uorescencequantitative PCR detection HBVDNA, 152 cases were positive, the positive rate was 93.8%, the average HBV-DNA concentration of 2.84 * 107; in 261 cases of HBs Ag, anti HBe, anti-HBcpositive specimens, real-time fl uorescence quantitative PCR detection of 128 cases were HBV-DNA positive, the positive rate was 64.3%, the average HBV-DNA concentration of 3.29 * 104.Detection of HBV-DNA by real time fl uorescent quantitative PCR conclusion can better refl ect the hepatitis B virus replication in vivo and the degree of infection.更多还原